Testing Results

From 2006.igem.org

(Difference between revisions)
Jump to: navigation, search
Line 13: Line 13:
</ul>
</ul>
-
[[University_of_Toronto_2006]]
+
back to [[University_of_Toronto_2006]]
== Completed Tests ==
== Completed Tests ==
Line 42: Line 42:
     <li>Module 1 (DH5a-z1): IPTG Induction of GFP
     <li>Module 1 (DH5a-z1): IPTG Induction of GFP
</ul>
</ul>
 +
 +
back to [[University_of_Toronto_2006]]
== Future Tests ==
== Future Tests ==
Line 49: Line 51:
     <li>Absorbance of Arabinose, and Abs/Emi Spectra of GFP/mRFP
     <li>Absorbance of Arabinose, and Abs/Emi Spectra of GFP/mRFP
</ol>
</ol>
 +
 +
back to [[University_of_Toronto_2006]]

Revision as of 03:10, 16 October 2006

Overview

We decided to integrate check-points into our construction plan, in order to test intermediary part functionality.
There are three basic modules:

  • Module 1: Arabinose inducible pBad/araC promoter (I0500) produces temperature-sensitive LacI (LacI ts), which then, upon dimerization, represses the IPTG inducible LacI+ pL promoter (R0011) resulting in a decrease in GFP (E0240)/mRFP (I13507)
  • Module 2: In Module 1, insert DNA sequence coding for 434 cI protein instead of the reporters (GFP/mRFP) and then add Prm+ promoter (I12006), which is repressed by 434 cI and add GFP
  • Module 3: Take Module 3 and add mRFP (J04450)

back to University_of_Toronto_2006

Completed Tests

Oct 8, 2006

  • Module 1 (DH5a): IPTG Induction of mRFP
  • Module 1 (DH5a): Arabinose Induction of LacI ts and repression of GFP

Oct 7, 2006

  • Module 1 (DH5a-z1): IPTG Induction of GFP
  • Module 1 (DH5a): Arabinose Induction of LacI ts and repression of GFP

Oct 5, 2006

  • Module 1 (DH5a-z1): Fluorescnece Images of GFP/mRFP

Sept 21, 2006

  • Module 1 (DH5a-z1): IPTG Induction of GFP/mRFP

Sept 16, 2006

  • Module 1 (DH5a-z1): IPTG Induction of GFP

back to University_of_Toronto_2006

Future Tests

  1. Surface response (GFP/mRFP) to varying IPTG and Arabinose
  2. Absorbance of Arabinose, and Abs/Emi Spectra of GFP/mRFP

back to University_of_Toronto_2006

Personal tools
Past/present/future years