Testing Results
From 2006.igem.org
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- | We decided to integrate check-points into our construction plan, in order to test intermediary part functionality. There are three basic modules: | + | == Overview == |
+ | |||
+ | We decided to integrate check-points into our construction plan, in order to test intermediary part functionality. | ||
+ | <br>There are three basic modules: | ||
<ul> | <ul> | ||
<li>'''Module 1:''' Arabinose inducible pBad/araC promoter (I0500) produces temperature-sensitive | <li>'''Module 1:''' Arabinose inducible pBad/araC promoter (I0500) produces temperature-sensitive | ||
Line 9: | Line 12: | ||
<li>'''Module 3:''' Take Module 3 and add mRFP (J04450) | <li>'''Module 3:''' Take Module 3 and add mRFP (J04450) | ||
</ul> | </ul> | ||
+ | |||
+ | back to [[University of Toronto 2006]] | ||
+ | |||
+ | == Completed Tests == | ||
+ | |||
+ | [[Oct 16, 2006]] | ||
+ | |||
+ | [[Oct 13, 2006]] | ||
+ | |||
+ | [[Oct 8, 2006]] | ||
+ | <ul> | ||
+ | <li>Module 1 (DH5a): IPTG Induction of mRFP | ||
+ | <li>Module 1 (DH5a): Arabinose Induction of LacI ts and repression of GFP | ||
+ | </ul> | ||
+ | |||
+ | [[Oct 7, 2006]] | ||
+ | <ul> | ||
+ | <li>Module 1 (DH5a-z1): IPTG Induction of GFP | ||
+ | <li>Module 1 (DH5a): Arabinose Induction of LacI ts and repression of GFP | ||
+ | </ul> | ||
+ | |||
+ | [[Oct 5, 2006]] | ||
+ | <ul> | ||
+ | <li>Module 1 (DH5a-z1): Fluorescnece Images of GFP/mRFP | ||
+ | </ul> | ||
+ | |||
+ | [[Sept 21, 2006]] | ||
+ | <ul> | ||
+ | <li>Module 1 (DH5a-z1): IPTG Induction of GFP/mRFP | ||
+ | </ul> | ||
+ | |||
+ | [[Sept 16, 2006]] | ||
+ | <ul> | ||
+ | <li>Module 1 (DH5a-z1): IPTG Induction of GFP | ||
+ | </ul> | ||
+ | |||
+ | back to [[University of Toronto 2006]] | ||
+ | |||
+ | == Future Tests == | ||
+ | |||
+ | <ol> | ||
+ | <li>Surface response (GFP/mRFP) to varying IPTG and Arabinose | ||
+ | <li>Absorbance of Arabinose, and Abs/Emi Spectra of GFP/mRFP | ||
+ | </ol> | ||
+ | |||
+ | back to [[University of Toronto 2006]] |
Latest revision as of 06:48, 17 October 2006
Overview
We decided to integrate check-points into our construction plan, in order to test intermediary part functionality.
There are three basic modules:
- Module 1: Arabinose inducible pBad/araC promoter (I0500) produces temperature-sensitive LacI (LacI ts), which then, upon dimerization, represses the IPTG inducible LacI+ pL promoter (R0011) resulting in a decrease in GFP (E0240)/mRFP (I13507)
- Module 2: In Module 1, insert DNA sequence coding for 434 cI protein instead of the reporters (GFP/mRFP) and then add Prm+ promoter (I12006), which is repressed by 434 cI and add GFP
- Module 3: Take Module 3 and add mRFP (J04450)
back to University of Toronto 2006
Completed Tests
- Module 1 (DH5a): IPTG Induction of mRFP
- Module 1 (DH5a): Arabinose Induction of LacI ts and repression of GFP
- Module 1 (DH5a-z1): IPTG Induction of GFP
- Module 1 (DH5a): Arabinose Induction of LacI ts and repression of GFP
- Module 1 (DH5a-z1): Fluorescnece Images of GFP/mRFP
- Module 1 (DH5a-z1): IPTG Induction of GFP/mRFP
- Module 1 (DH5a-z1): IPTG Induction of GFP
back to University of Toronto 2006
Future Tests
- Surface response (GFP/mRFP) to varying IPTG and Arabinose
- Absorbance of Arabinose, and Abs/Emi Spectra of GFP/mRFP
back to University of Toronto 2006