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- | ===12th July 2006===
| + | [[Image:encabezado3.jpg]] |
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- | The pH response over time was again measured but this time, we used liquid cultures which were already saturated (i.e. in the stationary phase). The results are shown below:
| + | '''Lab Work''' |
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- | ===11th July 2006===
| + | [[Image:lab work 3.jpg]] |
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- | Today we tested the timed pH response in 2 cell cultures: 1 with the LacZ gene, which was activated by IPTG, and one that had this gene absent.
| + | [[Lab work Schedule]] |
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- | ===10th July 2006===
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- | The colonies transformed on the 6th made it this time, and we isolated the plasmid DNA from three individual colonies for each biobrick. We also transformed some E. coli with
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- | {| border="1"
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- | | 23E || pSB1A3 || Plasmid || Plate 1 || AmpR
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- | |}
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- | to serve as an empty plasmid for the new LacZ and arsenic promoter/repressor parts which we will create.
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- | ===7th July 2006===
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- | [[Image:DSCN0576.JPG|256px|thumb|left|Acid Production with LacZ and Lactose]] | + | |
- | When bacteria with the lacZ gene inserted are present in a medium containing lactose, the pH does drop significantly.
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- | ===6th July 2006===
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- | Unfortunately the colonies we plated on the 4th did not survive due to a problem with the competent cells we used, so today we repeated transforming and plating colonies containing the following parts:
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- | {| border="1"
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- | | 9E || BBa_E0033 || LacZ alpha || Plate 2 || KanR
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- | | 1I || BBa_B0015 || Terminator || Plate 1 || AmpR
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- | | 7K || BBa_R0010 || IPTG responsive promoter || Plate 1 || AmpR
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- | | 3O || BBa_B0034 || RBS || Plate 1 || AmpR
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- | |}
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- | ===4th July 2006===
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- | We plated colonies containing plasmids with the following parts:
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- | {| border="1"
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- | | 9E || BBa_E0033 || LacZ alpha || Plate 2 || KanR
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- | | 3P || BBa_0010 || Terminator || Plate 2 || AmpR
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- | | 7K || BBa_R0010 || IPTG responsive promoter || Plate 1 || AmpR
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- | |}
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- | [http://2006.igem.org/Standard_Protocols Standard Protocols]
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- | [http://2006.igem.org/University_of_Edinburgh_2006 Main page]
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- | __NOTOC__
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