Lab Work

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===12th July 2006===
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[[Image:encabezado3.jpg]]
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The pH response over time was again measured but this time, we used liquid cultures which were already saturated (i.e. in the stationary phase).  The results will be put up soon.
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'''Lab Work'''
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A gel was run with the DNA isolated from the colonies transformed with the parts below, and they were isolated.
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[[Image:lab work 3.jpg]]
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===11th July 2006===
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[[Lab work Schedule]]
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Today we tested the timed pH response in 2 cell cultures:  1 with the LacZ gene, which was activated by IPTG, and one that had this gene absent.
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===10th July 2006===
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The colonies transformed on the 6th made it this time, and we isolated the plasmid DNA from three individual colonies for each biobrick. We also transformed some E. coli with
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{| border="1"
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| 23E || pSB1A3 || Plasmid || Plate 1 || AmpR
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|}
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to serve as an empty plasmid for the new LacZ and arsenic promoter/repressor parts which we will create.
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===7th July 2006===
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[[Image:DSCN0576.JPG|256px|thumb|left|Acid Production with LacZ and Lactose]]
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When bacteria with the lacZ gene inserted are present in a medium containing lactose, the pH does drop significantly.
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===6th July 2006===
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Unfortunately the colonies we plated on the 4th did not survive due to a problem with the competent cells we used, so today we repeated transforming and plating colonies containing the following parts:
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{| border="1"
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!width="40"|
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!width="100"|
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!width="70"|
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|-
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| 9E || BBa_E0033 || LacZ alpha || Plate 2 || KanR
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| 1I || BBa_B0015 || Terminator || Plate 1 || AmpR
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| 7K || BBa_R0010 || IPTG responsive promoter || Plate 1 || AmpR
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| 3O || BBa_B0034 || RBS || Plate 1 || AmpR
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|}
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===4th July 2006===
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We plated colonies containing plasmids with the following parts:
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{| border="1"
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!width="40"|
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!width="100"|
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!width="100"|
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!width="70"|
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|-
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| 9E || BBa_E0033 || LacZ alpha || Plate 2 || KanR
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|-
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| 3P || BBa_0010 || Terminator || Plate 2 || AmpR
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| 7K || BBa_R0010 || IPTG responsive promoter || Plate 1 || AmpR
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|}
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[http://2006.igem.org/Standard_Protocols Standard Protocols]
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[http://2006.igem.org/University_of_Edinburgh_2006 Main page]
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__NOTOC__
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Latest revision as of 04:17, 26 October 2006

Encabezado3.jpg

Lab Work

Lab work 3.jpg

Lab work Schedule

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