McGill University 2006

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<center>[[Image:Banner.gif]]</center>
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<small>See [http://www.flickr.com/search/?q=igem+montreal&m=text photos] from the [[ahessel:montreal|Ambassador visit]] on May 31, 2006.</small>
 
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= News =
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'''Welcome to the McGill Wiki!'''  
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* '''2006-06-15''' 1 month anniversary of attempting to ligate pGFP & Luciferase :) :)
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Basically, we're a team of 12 undergrads, 1 grad student, and 1 professor who like to have fun and clone things in our spare time. McGill University is located in Montreal, Quebec, which has given us wonderful opportunities to balance lab work with festivals and general craziness. iGEM has been a great opportunity to get lab experience while having the freedom to be creative, and we look forward to meeting the other teams at the jamboree!
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* '''2006-06-02''' Put up some profile pictures
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* '''2006-05-31''' [[iGEM Party Pictures!]]
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* '''2006-05-15''' Lab Notebook page created
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* '''2006-04-14''' Discussion page created
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* '''2006-03-16''' First kick-off meeting and team selection
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= Organisation =
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[[Image:Clip_image002.jpg]][[Image:Good_2.jpg]]
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== Projects ==
 
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[[Project 1]]
 
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[[Project 2]]
 
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[[Project 3]]
 
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== People ==
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=== Students ===
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|width=179.25px style="padding: 5px; background-color: #fffaf0; border: 2px solid #20b2aa;" |
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|[[Catie Lichten (Center for Nonlinear Dynamics)]]
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EDITED BY ASHWIN. If you want to change it, feel free :)-->
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|[[Octavio Mondragon (Physics)]]
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|[[Belinda Kong (Microbiology and Immunology)]]
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|[[Adrian Kaats (Biomedical Engineering)]]
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|[[Jamie Schafer (Microbiology and Immunology)]]
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|[[Horia Vulpe (Physiology)]]
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|[[Brock Dumville (Biomedical Sciences)]]
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|[[Ashwini Bapat (Biochemistry)]]
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|[[Aaron Lapierre (Anatomy & Cell Biology)]]
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|[[Josh Wright (Chemistry)]]
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|[[Jieun Kim(Biology)]]
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|[[Julia Ishak (Biomedical Sciences)]]
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|[[Ashwin Dixit (Microbiology and Immunology)]]
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|[[Adam Katolik (Biochemistry)]]
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|[[Alexandre David (Physiology)]]
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=== Supervisors ===
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<center><h2><font face="broadway,verdana">Projects</font></h2></center>
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|[[Jay Nadeau]]
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[[Elvis Pandzic]]
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|}
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== Timeline ==
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<table cellpadding="5" border="0"><tr>
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* [[Meetings]]
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<td valign="top" width="50%">
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* [[Lab Notebook]]
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<h3><ul><li>'''Team 1: Split YFP'''</ul></h3>
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* [[file registry]]
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This project on fluorescence complementation involves the joining of two proteins, Jun and Fos, each fused to a half terminus of YFP. Both of these chimeric proteins were fused to a beta gene that codes for a membrane protein. Then, two cell populations - one expressing Jun-beta-YFPN and the other Fos-beta-YFPC - were combined, ideally resulting in the fusion of the Jun and Fos proteins on the cell membrane. The two halves of the YFP protein would bind as well, giving rise to fluorescence.
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* [[iGEM Soundtrack]]
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== Tasks ==
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[[Background|Background]]
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* [[Task Assignment]]
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Modelling people: check out these bricks: BBa_I13920, BBa_I13921, BBa_I13922.
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[[Methods and Materials|Methods and Materials]]
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What happens if you plug these into your computer simulation?
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Also try this: BBa_J11002 and BBa_J06913. What happens if you substitute an AAV-degradation-tagged GFP?
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[[Results|Results]]
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Test EcoMscL to see if it does anything cool
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[[Future Prospects|Conclusions and Future Work]]
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How many vials of competent Mscl(-) E. coli do we have?  *make more*
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Do we have a luciferase reporter? What is its selection marker
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Amplify the Eco MscL plasmid
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==Journal Club==
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</td>
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==Useful papers==
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<td valign="top" width="50%">
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A synthetic gene–metabolic
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<h3><ul><li>''' Team 2: Repressilator'''</ul></h3>
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oscillator
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Eileen Fung1,2, Wilson W. Wong1, Jason K. Suen1, Thomas Bulter1,
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Sun-gu Lee1 & James C. Liao1,2
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NATURE | VOL 435 | 5 MAY 2005
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http://www.nature.com/nature/journal/v435/n7038/abs/nature03508.html
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Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):679-84.
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Our project is based on the repressilator system coupled to quorum sensing as described by Jordi Garcia-Ojalvo, Michael B. Elowitz and Steven H. Strogatz in "Modeling a synthetic multicellular clock: Repressilators coupled by quorum sensing" (PNAS). We attempt to visualize the synchronization of the oscillatory phase between cells by the addition of the CFP reporter gene. We expand on this theory by placing cI under the control of pLac, hoping that this would assist in synchronizing the oscillations.
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Synchronizing genetic relaxation oscillators by intercell signaling.
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McMillen D, Kopell N, Hasty J, Collins JJ.
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http://www.pnas.org/cgi/content/full/99/2/679
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[[Theory Behind the Oscillator|Theory Behind the Oscillator]]
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Cell Mol Life Sci. 2006 May
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[[McGill_Repressilator_M_and_M|Methods and Materials]]
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The design of intracellular oscillators that interact with metabolism.
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Wong WW, Liao JC.
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== Parts shopping list==
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[[McGill_Repressilator_Results|Results]]
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</td>
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[[Parts Shopping List]]
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</tr></table>
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[[Example for oscillator]]
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<center><h2><font face="broadway,verdana">Lab Procedures</font></h2></center>
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<table border="0"><tr><td valign="top" width="85%">
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* [[Protocols]]
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* [[Lab Notebook]]
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</td>
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<td>[[Image:Test_tubes.jpg]]</td></tr></table>
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|-valign="top"
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<center><h2><font face="broadway,verdana">Club</font></h2></center>
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* [[News]]
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* [[Team Members]]
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* [[Executive Council|Executive Council]]
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* [[Journal Club Meetings]]
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* [[Journal Club Papers]]
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<center><h2><font face="broadway,verdana">Just for Fun</font></h2></center>
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* [[iGEM Party Pictures]]
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* [[ahessel:montreal|Ambassador Visit]] [http://www.flickr.com/search/?q=igem+montreal&m=text Photos]
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* [[iGEM Soundtrack]]
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<Center>[[Image:Poutine.gif]]</Center>
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"Introduction" is now under the Discussion page
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__NOTOC__

Latest revision as of 20:19, 31 October 2006

Banner.gif


Welcome to the McGill Wiki! Basically, we're a team of 12 undergrads, 1 grad student, and 1 professor who like to have fun and clone things in our spare time. McGill University is located in Montreal, Quebec, which has given us wonderful opportunities to balance lab work with festivals and general craziness. iGEM has been a great opportunity to get lab experience while having the freedom to be creative, and we look forward to meeting the other teams at the jamboree!

Clip image002.jpgGood 2.jpg



Projects

  • Team 1: Split YFP

This project on fluorescence complementation involves the joining of two proteins, Jun and Fos, each fused to a half terminus of YFP. Both of these chimeric proteins were fused to a beta gene that codes for a membrane protein. Then, two cell populations - one expressing Jun-beta-YFPN and the other Fos-beta-YFPC - were combined, ideally resulting in the fusion of the Jun and Fos proteins on the cell membrane. The two halves of the YFP protein would bind as well, giving rise to fluorescence.

Background

Methods and Materials

Results

Conclusions and Future Work

  • Team 2: Repressilator

Our project is based on the repressilator system coupled to quorum sensing as described by Jordi Garcia-Ojalvo, Michael B. Elowitz and Steven H. Strogatz in "Modeling a synthetic multicellular clock: Repressilators coupled by quorum sensing" (PNAS). We attempt to visualize the synchronization of the oscillatory phase between cells by the addition of the CFP reporter gene. We expand on this theory by placing cI under the control of pLac, hoping that this would assist in synchronizing the oscillations.

Theory Behind the Oscillator

Methods and Materials

Results

Lab Procedures

Test tubes.jpg

Club

Just for Fun

Poutine.gif
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