McGill University 2006

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=''NAME TBA (we are not clever enough today)''=
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<center>[[Image:Banner.gif]]</center>
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= News =
 
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* '''2006-03-16''' First kick-off meeting and team selection
 
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= Organisation =
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'''Welcome to the McGill Wiki!'''
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Basically, we're a team of 12 undergrads, 1 grad student, and 1 professor who like to have fun and clone things in our spare time. McGill University is located in Montreal, Quebec, which has given us wonderful opportunities to balance lab work with festivals and general craziness. iGEM has been a great opportunity to get lab experience while having the freedom to be creative, and we look forward to meeting the other teams at the jamboree!
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== People ==
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[[Image:Clip_image002.jpg]][[Image:Good_2.jpg]]
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=== Students ===
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|[[Catie Lichten (Center for Nonlinear Dynamics)]]
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|[[Octavio Mondragon (Physics)]]
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|[[Belinda Kong (Microbiology and Immunology)]]
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|[[Adrian Kaats (Biomedical Engineering)]]
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|[[Jamie Schafer (Microbiology and Immunology)]]
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|[[Josh Wright (Chemistry)]]
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=== Supervisors ===
 
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|[[Jay Nadeau]]
 
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== Timeline ==
 
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* [[Meetings]]
 
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== Tasks ==
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* [[Task Assignment]]
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EDITED BY ASHWIN. If you want to change it, feel free :)-->
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=Introduction= Our brainstorming meeting on 3-16 gave us several preliminary ideas which we will explore via modeling and research
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<center><h2><font face="broadway,verdana">Projects</font></h2></center>
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<h3><ul><li>'''Team 1: Split YFP'''</ul></h3>
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This project on fluorescence complementation involves the joining of two proteins, Jun and Fos, each fused to a half terminus of YFP. Both of these chimeric proteins were fused to a beta gene that codes for a membrane protein. Then, two cell populations - one expressing Jun-beta-YFPN and the other Fos-beta-YFPC - were combined, ideally resulting in the fusion of the Jun and Fos proteins on the cell membrane. The two halves of the YFP protein would bind as well, giving rise to fluorescence.
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[[Background|Background]]
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[[Methods and Materials|Methods and Materials]]
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[[Results|Results]]
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[[Future Prospects|Conclusions and Future Work]]
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</td>
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<td valign="top" width="50%">
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<h3><ul><li>''' Team 2: Repressilator'''</ul></h3>
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Our project is based on the repressilator system coupled to quorum sensing as described by Jordi Garcia-Ojalvo, Michael B. Elowitz and Steven H. Strogatz in "Modeling a synthetic multicellular clock: Repressilators coupled by quorum sensing" (PNAS). We attempt to visualize the synchronization of the oscillatory phase between cells by the addition of the CFP reporter gene. We expand on this theory by placing cI under the control of pLac, hoping that this would assist in synchronizing the oscillations.
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[[Theory Behind the Oscillator|Theory Behind the Oscillator]]
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[[McGill_Repressilator_M_and_M|Methods and Materials]]
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[[McGill_Repressilator_Results|Results]]
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</td>
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</tr></table>
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<center><h2><font face="broadway,verdana">Lab Procedures</font></h2></center>
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<table border="0"><tr><td valign="top" width="85%">
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* [[Protocols]]
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* [[Lab Notebook]]
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</td>
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<td>[[Image:Test_tubes.jpg]]</td></tr></table>
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<center><h2><font face="broadway,verdana">Club</font></h2></center>
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* [[News]]
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* [[Team Members]]
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* [[Executive Council|Executive Council]]
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* [[Journal Club Meetings]]
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* [[Journal Club Papers]]
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<center><h2><font face="broadway,verdana">Just for Fun</font></h2></center>
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* [[iGEM Party Pictures]]
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* [[ahessel:montreal|Ambassador Visit]] [http://www.flickr.com/search/?q=igem+montreal&m=text Photos]
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* [[iGEM Soundtrack]]
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<Center>[[Image:Poutine.gif]]</Center>
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__NOTOC__

Latest revision as of 20:19, 31 October 2006

Banner.gif


Welcome to the McGill Wiki! Basically, we're a team of 12 undergrads, 1 grad student, and 1 professor who like to have fun and clone things in our spare time. McGill University is located in Montreal, Quebec, which has given us wonderful opportunities to balance lab work with festivals and general craziness. iGEM has been a great opportunity to get lab experience while having the freedom to be creative, and we look forward to meeting the other teams at the jamboree!

Clip image002.jpgGood 2.jpg



Projects

  • Team 1: Split YFP

This project on fluorescence complementation involves the joining of two proteins, Jun and Fos, each fused to a half terminus of YFP. Both of these chimeric proteins were fused to a beta gene that codes for a membrane protein. Then, two cell populations - one expressing Jun-beta-YFPN and the other Fos-beta-YFPC - were combined, ideally resulting in the fusion of the Jun and Fos proteins on the cell membrane. The two halves of the YFP protein would bind as well, giving rise to fluorescence.

Background

Methods and Materials

Results

Conclusions and Future Work

  • Team 2: Repressilator

Our project is based on the repressilator system coupled to quorum sensing as described by Jordi Garcia-Ojalvo, Michael B. Elowitz and Steven H. Strogatz in "Modeling a synthetic multicellular clock: Repressilators coupled by quorum sensing" (PNAS). We attempt to visualize the synchronization of the oscillatory phase between cells by the addition of the CFP reporter gene. We expand on this theory by placing cI under the control of pLac, hoping that this would assist in synchronizing the oscillations.

Theory Behind the Oscillator

Methods and Materials

Results

Lab Procedures

Test tubes.jpg

Club

Just for Fun

Poutine.gif


Personal tools
Past/present/future years