AnswerA
From 2006.igem.org
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So it would seem we could in fact, alter the amount of negative (-)supercoiling. | So it would seem we could in fact, alter the amount of negative (-)supercoiling. | ||
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+ | Also, Dr. Eckdahl found that Novaviacin could also be used as well to the same effect. |
Latest revision as of 16:33, 30 May 2006
See link: [http://www.jbc.org/cgi/reprint/272/29/18434] Hin-mediated Inversion on Positively Supercoiled DNA
Hin-recombinase requires negatively(-) supercoiled DNA for an efficient inversion. Results Section of Paper: Using plasmid pKH336, they introduced Chloroquine, a DNA-intercalating agent that unwinds the double helix upon binding. The paper goes on to say that Lk (sum of writhe or superhelical turns and twist); the unwinding effect of chloroquine-binding lowers the twist of the plasmid. The result is writhe increases because Lk does not change unless the backbone of DNA is broken and rejoined. Thus, a certain amount of chloroquine will remove supercoiling (relaxation) from (-)pKH336 and adds more supercoiling to (+)pKH336.
So it would seem we could in fact, alter the amount of negative (-)supercoiling.
Also, Dr. Eckdahl found that Novaviacin could also be used as well to the same effect.