Davidson Parts
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| - | + | '''''Hin gene''''' | |
| - | Forward primer | + | Primers for Hin gene the 72nd base was mutated from a "T" to a "C" in order to remove a naturally occuring SpeI site. The mutatation is in '''bold'''. |
| - | 5’ | + | |
| + | '''Forward primer''' | ||
| + | |||
| + | 5’ gcattctagaatggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttac'''C'''agtgcaaattg 3’ | ||
| + | |||
| + | '''Reverse primer''' | ||
| - | |||
5’ atgcctgcaggcggccgcactagtttaattcattcgtttttttatac 3’ | 5’ atgcctgcaggcggccgcactagtttaattcattcgtttttttatac 3’ | ||
| + | |||
| + | |||
| + | '''''Recombinational Enhancer''''' | ||
| + | |||
| + | These are the olioges that we ordered to make the recombinational enhancer. | ||
| + | |||
| + | AATTCGCGGCCGCTTCTAGATTCGGGTGTCAACAATTGACCAAAATAT | ||
| + | |||
| + | CGATTTACAGCGTAATGCGCTTTCTAGTGCAAATTGTGACCGC | ||
| + | |||
| + | ATTTTACTAGTTGCGGCCGCCTGCA | ||
| + | |||
| + | GGCGGCCGCAACTAGTAAAATGCGGTCACAATTTGCACTAGAA | ||
| + | |||
| + | AGCGCATTACGCTGTAAATCGATATTTTGGTCAATTGTTGACACCCGAATCTAGAAGCGGCCGCG | ||
Revision as of 19:58, 6 June 2006
Hin gene
Primers for Hin gene the 72nd base was mutated from a "T" to a "C" in order to remove a naturally occuring SpeI site. The mutatation is in bold.
Forward primer
5’ gcattctagaatggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttacCagtgcaaattg 3’
Reverse primer
5’ atgcctgcaggcggccgcactagtttaattcattcgtttttttatac 3’
Recombinational Enhancer
These are the olioges that we ordered to make the recombinational enhancer.
AATTCGCGGCCGCTTCTAGATTCGGGTGTCAACAATTGACCAAAATAT
CGATTTACAGCGTAATGCGCTTTCTAGTGCAAATTGTGACCGC
ATTTTACTAGTTGCGGCCGCCTGCA
GGCGGCCGCAACTAGTAAAATGCGGTCACAATTTGCACTAGAA
AGCGCATTACGCTGTAAATCGATATTTTGGTCAATTGTTGACACCCGAATCTAGAAGCGGCCGCG
