Davidson Parts

From 2006.igem.org

(Difference between revisions)
Jump to: navigation, search
Line 11: Line 11:
5’ atgcctgcaggcggccgcactagtttaattcattcgtttttttatac  3’
5’ atgcctgcaggcggccgcactagtttaattcattcgtttttttatac  3’
 +
'''''Hin gene with LVA degredation tag'''''
 +
Will have the following primers synthesized so that the Hin gene has a LVA degredation tag.
 +
 +
'''Forward Primer'''
 +
*Consists of a <font color='green'>BioBrick prefix</font color>, <font color='blue'>four base pairs from plasmid pSB1A2 [http://partsregistry.org/Part:pSB1A2]</font color>, and<font color='orange'> the first three base pairs of the Hin gene</font color>
 +
<font face="courier new"><font color='blue'>TCTG</font color><font color='green'>GAATTCGCGGCCGCATCTAGAG</font color><font color='orange'>ATG</font color></font face>
 +
 +
'''''Reverse Primer'''''
 +
*Consists of the <font color='green'>Bio Brick suffix</font color>,<font color='red'> Stop codon</font color>, <font color='purple'>LVA tag</font color> and the ,<font color='orange'>last 20 base pairs of the Hin gene</font color> (without the stop codon)
 +
 +
<font face="courier new"><font color='green'>CTGCAGGCGGCCGCTACTAGT</font color><font color='red'>ATT</font color><font color='purple'>AAGCTACTAAAGCGTAGTTTTCGTCGTTTGCAGC</font color><font color='orange'>ATTCATTCGTTTTTTTATAC</font color></font face>
 +
 +
 +
[Recombinational Enahancer: Part BBa_J3101]
'''''Recombinational Enhancer: Part BBa_J3101'''''
'''''Recombinational Enhancer: Part BBa_J3101'''''

Revision as of 18:29, 12 June 2006

Hin gene: Part BBa_J31000

Primers for Hin gene the 72nd base was mutated from a "T" to a "C" in order to remove a naturally occuring SpeI site. The mutatation is in bold.

Forward primer

5’ gcattctagaatggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttacCagtgcaaattg 3’

Reverse primer

5’ atgcctgcaggcggccgcactagtttaattcattcgtttttttatac 3’

Hin gene with LVA degredation tag

Will have the following primers synthesized so that the Hin gene has a LVA degredation tag.

Forward Primer

  • Consists of a BioBrick prefix, four base pairs from plasmid pSB1A2 [http://partsregistry.org/Part:pSB1A2], and the first three base pairs of the Hin gene

TCTGGAATTCGCGGCCGCATCTAGAGATG

Reverse Primer

  • Consists of the Bio Brick suffix, Stop codon, LVA tag and the ,last 20 base pairs of the Hin gene (without the stop codon)

CTGCAGGCGGCCGCTACTAGTATTAAGCTACTAAAGCGTAGTTTTCGTCGTTTGCAGCATTCATTCGTTTTTTTATAC


[Recombinational Enahancer: Part BBa_J3101] Recombinational Enhancer: Part BBa_J3101

These are the oligos that we ordered to make the recombinational enhancer.

AATTCGCGGCCGCTTCTAGATTCGGGTGTCAACAATTGACCAAAATAT

CGATTTACAGCGTAATGCGCTTTCTAGTGCAAATTGTGACCGC

ATTTTACTAGTTGCGGCCGCCTGCA

GGCGGCCGCAACTAGTAAAATGCGGTCACAATTTGCACTAGAA

AGCGCATTACGCTGTAAATCGATATTTTGGTCAATTGTTGACACCCGAATCTAGAAGCGGCCGCG

Personal tools
Past/present/future years