Hin gene: Part BBa J31000
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'''''Hin gene: Part BBa_J31000'''''[http://partsregistry.org/Part:BBa_J31000] | '''''Hin gene: Part BBa_J31000'''''[http://partsregistry.org/Part:BBa_J31000] | ||
| - | Primers for Hin gene | + | Primers for Hin gene |
| + | * The 72nd base was mutated from a "T" to a "C" in order to remove a naturally occuring SpeI site. The mutatation is in <font color='red'>red</font color>. | ||
'''Forward primer''' | '''Forward primer''' | ||
Revision as of 18:54, 12 June 2006
Hin gene: Part BBa_J31000[http://partsregistry.org/Part:BBa_J31000]
Primers for Hin gene
- The 72nd base was mutated from a "T" to a "C" in order to remove a naturally occuring SpeI site. The mutatation is in red.
Forward primer
5’ GCATTCTAGAATGGCTACTATTGGGTATATTCGGGTGTCAACAATTGACCAAAATATCGATTTACAGCGTAATGCGCTTACCAGTGCAAATTG 3’
Reverse primer
5’ ATGCCTGCAGGCGGCCGCACTAGTTTAATTCATTCGTTTTTTTATAC 3’
