ETH2006 iptg

From 2006.igem.org

Revision as of 08:05, 25 October 2006 by Dimo (Talk | contribs)
Jump to: navigation, search

Contents

chemical sensing device

The chemical sensing device's PoPs output activity should be a monotonic function of the concentration of a certain substance in the cell's environment.

implementation alternatives

  1. Lactate lacI represses, IPTG induces ([http://partsregistry.org/Part:BBa_R0011 BBa_R0011] or [http://partsregistry.org/Part:BBa_R0010 BBa_R0010] )
  2. Tetracycline, TetR inhibitor, Tet inducer by inhibiting TetR (or aTc, it's analog) ([http://partsregistry.org/Part:BBa_R0040 BBa_R0040])
  3. combination thereof ([http://partsregistry.org/Part:BBa_I13614 BBa_I13614] / [http://partsregistry.org/Part:BBa_I13617 BBa_13617] / [http://partsregistry.org/Part:BBa_I13623 BBa_I13623] / [http://partsregistry.org/Part:BBa_I13624 BBa_I13624] / [http://partsregistry.org/Part:BBa_I13627 BBa_I13627] / [http://partsregistry.org/Part:BBa_I13637 BBa_I13637] / [http://partsregistry.org/Part:BBa_I13653 BBa_I13653])
  4. simple sugar Arabinose ([http://partsregistry.org/Part:BBa_R0080 BBa_R0080])

I see the main difficulty in the spatial separation as the cells are growing in the petri dishes. since the inducers are water-soluble we would have to fix the chemicals onto the petri dish.

complete system based on alternative 1

assembly procedure

where we got it and link to theyer documentation

Progress:

2006/10/03
Made LB-Agar plates with antibiotics
2006/10/04
Transformed cells, plated them
2006/10/05
Found plates empty after 18h on the table, put into incubator at 37°C
2006/10/06
picked cultures onto fresh plates

test procedure

test results

parts

back to main page

Personal tools
Past/present/future years