Brown:Projects
From 2006.igem.org
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Bacterial Freeze Tag
Overview
Sender Cell
AHL Sensor
'Freeze Machine'
A promoter that is regulated by LacI will promote the production of LasI, MotB, and cI. This will subsequently inhibit the production of CFP and LasR. In the presence of LacI, however, MotB, LasI, and cI will not be produced. CFP will therefore be produced along with LasR and LacI. This results in the "freezing" of the cell.
Unfreeze Mechanism
Magnetotactic Bacteria
Avadin-Biotin Complex
To create magnetosomes covered in Avadin. The basic idea is to create a fusion protein from MagA and Avadin, then isolate the magnetic particles, which will now be coated in avadin. Avadin forms one of the strongest bindings known with the molecule biotin.
Bayer, E. A. and Wilchek, M. (1978). The avidin-biotin complex as a tool in molecular biology. Trends Biochem. Sci. 3, N237-N239.
http://www.weizmann.ac.il/Biological_Chemistry/scientist/Bayer/BayerAvidin.html great review
http://www.avidity.com/apps.html current applications of tagging biotin
http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=92791044 Artifical synthesized Gene coding for avidin
