Balloon Bacteria : Chiba 2006

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Contents

Concept

Balloonconcept.jpg

The light in the morning leaking from a gap between the curtains... this morning lights calls and awakes the E. coli.

Suprise!!

The E. coli become bigger and bigger like BALLOON!!!

→World first bacteria that swells by lights.!!!

When growing...

The movement of the E. coli can be obvered with the naked eyes !

"Big" E. coli is easily sorted by FACS !

Make much more proteins/cell !

Design

First

The E. coli doesn't have photoreceptor. But...

We have Cph8(utwxas/uCSF;iGEM2005)in our hand

Second

The E. coli divides the cells by forming "partition". So...

'Expression of the enzyme in septem formation is knocked-down.

Third

E. coli rather "extend" due to the action of RodA. So...

We make inducible RodAmut.

Design3.jpg



Experiments

  1. We made inducible rodAmut.
  2. We made CI inverter and compared it.


Results

1.rodAmut

RodAmut.jpg

...show more.[[rodAmut]]

2.CI inverter

CIinverter2.jpg

...show more.CI inverter

Member

Chiba 2006 http://chem.tf.chiba-u.jp/igem/ iGEM Chiba

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