Construction

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(October 14, 2006)
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<font size=4>< [[Aug11-Sep7]] | [[Sep8-14]] | [[Sep15-26]] | [[Sep27-Oct3]] | [[Oct4-Oct13]] | [[Construction]] ></font>
<font size=4>< [[Aug11-Sep7]] | [[Sep8-14]] | [[Sep15-26]] | [[Sep27-Oct3]] | [[Oct4-Oct13]] | [[Construction]] ></font>
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== October 18, 2006 ==
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'''Andy, Konstantin:'''
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<ul>
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    <li>Transformed and plated Q03400 (2005) (2006)
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    <li>Gel-extracted UT1 DEF with EcoRI/SpeI (2500-3000, 5000, 8000), 8000 band bright, digestion appeared incomplete
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    <li>Made A, K, AK plates
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    <li>Made o/n of Q04400 (2005) (2006) Q04510 (2005) (2006)
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    <li>Tested UT3 in DH5a with 0%-2% arabinose
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</ul>
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'''To-Do List:'''
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<ul>
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    <li>Make o/n of Q03400 (2005) (2006)
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    <li>Replate UT1 with miniprep from a good conlony
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    <li>More ligation?
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</ul>
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 +
[http://2006.igem.org/University_of_Toronto_2006 Home]
== October 14, 2006 ==
== October 14, 2006 ==

Revision as of 02:33, 19 October 2006

< Aug11-Sep7 | Sep8-14 | Sep15-26 | Sep27-Oct3 | Oct4-Oct13 | Construction >

October 18, 2006

Andy, Konstantin:

  • Transformed and plated Q03400 (2005) (2006)
  • Gel-extracted UT1 DEF with EcoRI/SpeI (2500-3000, 5000, 8000), 8000 band bright, digestion appeared incomplete
  • Made A, K, AK plates
  • Made o/n of Q04400 (2005) (2006) Q04510 (2005) (2006)
  • Tested UT3 in DH5a with 0%-2% arabinose

To-Do List:

  • Make o/n of Q03400 (2005) (2006)
  • Replate UT1 with miniprep from a good conlony
  • More ligation?

[http://2006.igem.org/University_of_Toronto_2006 Home]

October 14, 2006

Charles, Jovan:

  • Mini-prepped UT2 2/4 and UT3 7
  • Transform UT2 2/4 and UT3 7 into DH5a-z1 and DH5a cells
  • Continue with the IPTG test for the rest of the colonies that we weren’t able to test yesterday

To-Do List:

  • Measure absorbance spectrum of Arabinose
  • Make o/n of working UT2/UT3 for repeat of IPTG test.
  • Look into using tetR and tet pL instead of cI and Prm+

[http://2006.igem.org/University_of_Toronto_2006 Home]

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