Davidson 2006

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(<font color='cyan'><font face="chalkboard">Current Course of Action</font face></font color>)
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* We decided to test the size constraints first, so we are building stacks of pancakes only one high at first. We will try to flip the promoter (smaller) in one case, and the coding region (bigger) in another case. The coding region is Tet<sup>R</sup> resistance gene isolated from a plasmid in the Registry. For higher stacks of pancakes, we will also use chloramphenicol<sup>R</sup> and cycloheximide<sup>R</sup>.
* We decided to test the size constraints first, so we are building stacks of pancakes only one high at first. We will try to flip the promoter (smaller) in one case, and the coding region (bigger) in another case. The coding region is Tet<sup>R</sup> resistance gene isolated from a plasmid in the Registry. For higher stacks of pancakes, we will also use chloramphenicol<sup>R</sup> and cycloheximide<sup>R</sup>.
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== '''Assembly Plan'''==
 
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[[Western Assembly Plan]]
 
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[[Davidson Assembly Plan]]
 
== <font face="chalkboard"><font color='blue'> Students</font color> ==
== <font face="chalkboard"><font color='blue'> Students</font color> ==
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* <font face="chalkboard"> [[Assembly powerpoint]]</font face>
* <font face="chalkboard"> [[Assembly powerpoint]]</font face>
*[[Assembly plan]]
*[[Assembly plan]]
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*Final Assembly plans:
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**[[Western Assembly Plan]]
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**[[Davidson Assembly Plan]]
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== <font color='cyan'><font face="chalkboard">Current Course of Action</font face></font color>==  
== <font color='cyan'><font face="chalkboard">Current Course of Action</font face></font color>==  

Revision as of 14:56, 2 August 2006

Logo.gif


[http://www.bio.davidson.edu/courses/synthetic/photos/FlapJack_HotCakes.html Official Team Photo (rollover image)]

DC team.JPG Logo
Malcolm, Laurie, Sabriya, Erin, Lance


Contents

Project

Who: The Mammoths, and FlapJack and the Pancakes

What: Our goal is to solve the Pancake problem[http://www.cut-the-knot.org/SimpleGames/Flipper.shtml] in a biological system.

When: Summer and Fall '06

Where: Missouri Western State University and Davidson College

Why: Because we love breakfast... well most of us.

How: Trying to invert antibiotic resistance genes using the Hin/Hix inversion method found in Salmonella typhimurium.


  • Our overall goal was to see if we could use synthetic methods to construct an E. coli computer. If we grew 1015 cells, and each cell was attempting to solve the problem, then even rare events might be solvable by E. coli computers.
  • We decided to tackle the burnt pancake problem since DNA, like burnt pancakes, has a directionality. We needed a way to flip the DNA and chose the Hin/Hix system. We had to isolate RE, HixC from S. typhimurium strain XX (need to insert name), and Hin from S. typhimurium, strain TA100 .
  • We decided to test the size constraints first, so we are building stacks of pancakes only one high at first. We will try to flip the promoter (smaller) in one case, and the coding region (bigger) in another case. The coding region is TetR resistance gene isolated from a plasmid in the Registry. For higher stacks of pancakes, we will also use chloramphenicolR and cycloheximideR.


Students

  • Erin Zwack is a rising junior biology major at Davidson College.
  • Lance Harden is a rising sophomore at Davidson College who will probably major in math.
  • Samantha Simpson is a rising sophomore at Davidson College who might design a major in genomics.

Faculty

  • Laurie J. Heyer [http://www.davidson.edu/math/heyer/ Department of Mathematics]
  • Karmella Haynes [http://www.bio.davidson.edu/ Department of Biology - teaching postdoc and visiting assistant professor]

Papers of Interest

Parts Created

Pancake Parts

Questions

Assembly Issues


Current Course of Action

Plasmids and Bacteria     Hix and RE    Hin and Antibiotic Pancakes    Progress

Pancake Simulators aka The Lancelator

MATLAB Code

[http://gcat.davidson.edu/IGEM06/randompancakeflipper.m User-Friendly Version]
[http://gcat.davidson.edu/IGEM06/powerflipper.m Power-User Version]
[http://gcat.davidson.edu/IGEM06/powerflipperplus.m Power-User Update]
[http://gcat.davidson.edu/IGEM06/findmin.m Findmin]
[http://gcat.davidson.edu/IGEM06/bigtrial.m Bigtrial]
[http://gcat.davidson.edu/IGEM06/pancakeplotter.m Pancakeplotter]
[http://gcat.davidson.edu/IGEM06/permplotter.m Permplotter]
[http://gcat.davidson.edu/IGEM06/bioperms.m Bioperms]
[http://gcat.davidson.edu/IGEM06/signedperms.m Signedperms]
[http://gcat.davidson.edu/IGEM06/adjmat.m Adjmat]
[http://gcat.davidson.edu/IGEM06/make_radial.m Make_radial]
[http://gcat.davidson.edu/IGEM06/find_diam.m Find_diam]

Personal tools
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