Davidson 2006

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('''Assembly Issues''')
(Plasmids)
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=='''Current Course of Action'''==  
=='''Current Course of Action'''==  
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===Plasmids===
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[[Plasmids and Bacteria]]
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*'''pSB1A3'''
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***amp resistant
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*** copy number:100-300 
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***size:2157 empty
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*'''pSB1AC3'''
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*** part-BBAp1000
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***copy number:100-3000
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***amp and CmR resistant
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***contains device that provides CmR
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***size:3844 ( with device) 3005 (without device)
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*'''pSB1AK3'''
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***part BBa_|13535
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***copy number:100-300
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***amp and kan resistance
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***contains a device for screening test
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***size: 3457 (with device) 3189 (without device)
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* Transform bacteria  '''We are using ''Salmonella typhimurium'' for the Hin protein as it was the referenced species in all of the literature.'''
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* Create RE biobrick (completed)
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*Isolating genomic DNA from Salmonella typhimurium
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*Ordered olioges for recombinational enhancer and primers for the hin gene
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==Pancake Simulator aka The Lancelator==
==Pancake Simulator aka The Lancelator==
[[randompancakeflipper.m]]
[[randompancakeflipper.m]]

Revision as of 19:53, 6 June 2006

Logo.gif

DC team.JPG
Malcolm, Laurie, Sabriya, Erin, Lance.


Contents

Students

• Sabriya Rosemond [1] is a rising junior biology major at Hampton University in VA.

• Erin Zwack [2] is a rising junior biology major at Davidson College in NC.

• Lance Harden [3] is a rising sophomore at Davidson College, who might major in math.


Faculty

• A. Malcolm Campbell [http://www.bio.davidson.edu/campbell Department of Biology], [4]

• Laurie J. Heyer [http://www.davidson.edu/math/heyer/ Department of Mathematics], [5]

Papers of Interest

• [http://www.bio.davidson.edu/courses/synthetic/papers/Sanders_04.pdf Stepwise Dissection of the Hin-catalyzed Recombination Reaction from Synapsis to Resolution. Erin R. Sanders and Reid C. Johnson]

• [http://www.molcells.org/home/journal/article_read.asp?volume=16&number=3&startpage=377 The Effects of Ethidium Bromide and Mg++ Ion on Strand Exchange in the Hin-mediated Inversion Reaction. Hee Jung Lee et al.] Excellent description of Hin recombination mechanism.

• [http://www.jbc.org/cgi/reprint/267/16/11183 The Effects of Symmetrical Recombination Site hixC on Hin Recombinase Function. Heon Man Lim et al.]

• [http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=3457367 The role of the loxP spacer region in P1 site-specific recombination. Ronald H.Hoess et al.]

• [http://www.biomedcentral.com/1471-2164/7/73 A high-throughput screen identifying sequence and promiscuity characteristics of the loxP spacer region in Cre-mediated recombination. Perseus I Missirlis et al.]

• [http://nar.oxfordjournals.org/cgi/content/full/30/13/2764 Non-contact positions impose site selectivity on Cre recombinase. Andreas W. Rufer and Brian Sauer]

• [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=103771 Growth Phase-Dependent Variation in Protein Composition of the Escherichia coli Nucleoid]

• [http://www.jbc.org/cgi/content/full/272/29/18434 Hin-mediated Inversion on Positively Supercoiled DNA]

• [http://jb.asm.org/cgi/content/full/185/3/1097 Plasmid DNA Supercoiling and Gyrase Activity in Escherichia coli Wild-Type and rpoS Stationary-Phase Cells]


Project Description



E hop.jpg



Parts Created

Davidson Parts

Questions

General Questions

Math Questions

Biology Questions

Assembly Issues

The What's and How's

Current Course of Action

Plasmids and Bacteria

Pancake Simulator aka The Lancelator

randompancakeflipper.m

Personal tools
Past/present/future years