The What's and How's
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*<u>ERIN and SABRIYA</u> Which DNA parts exist in the registry?[[Answer1]][[More Parts]]<br> | *<u>ERIN and SABRIYA</u> Which DNA parts exist in the registry?[[Answer1]][[More Parts]]<br> | ||
* <u>ERIN and SABRIYA</u>Which DNA parts will have to be designed? Will they be synthesized or produced by PCR? <br> | * <u>ERIN and SABRIYA</u>Which DNA parts will have to be designed? Will they be synthesized or produced by PCR? <br> | ||
| - | [[loxP sites] | + | [[loxP sites][ [[Hin/Hix]] [[CRE gene]] [[Degradation Tags]] |
# [http://www.bio.davidson.edu/courses/Molbio/Protocols/reagents.html Common molecular reagents] | # [http://www.bio.davidson.edu/courses/Molbio/Protocols/reagents.html Common molecular reagents] | ||
| + | # [http://partsregistry.org/Assembly:Standard_assembly Standard Assembly] | ||
# [http://www.bio.davidson.edu/courses/Molbio/Protocols/anneal_oligos.html Building dsDNA with Oligos] | # [http://www.bio.davidson.edu/courses/Molbio/Protocols/anneal_oligos.html Building dsDNA with Oligos] | ||
| - | # [http://www.bio.davidson.edu/courses/Molbio/Protocols/pcr.html Setting up PCR mixtures | + | # [http://www.bio.davidson.edu/courses/Molbio/Protocols/pcr.html Setting up PCR mixtures] |
# [http://www.bio.davidson.edu/courses/Molbio/Protocols/magnesium.html PCR and Mg<sup>2+</sup> concentration] | # [http://www.bio.davidson.edu/courses/Molbio/Protocols/magnesium.html PCR and Mg<sup>2+</sup> concentration] | ||
# [http://www.bio.davidson.edu/courses/Molbio/Protocols/pourgel.html Pouring an agarose gel] | # [http://www.bio.davidson.edu/courses/Molbio/Protocols/pourgel.html Pouring an agarose gel] | ||
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# [http://www.bio.davidson.edu/courses/Molbio/Protocols/gelpure.html ElectroElute Gel Purification] | # [http://www.bio.davidson.edu/courses/Molbio/Protocols/gelpure.html ElectroElute Gel Purification] | ||
# [http://www.bio.davidson.edu/courses/Molbio/Protocols/ligation.html Ligation Protocol] | # [http://www.bio.davidson.edu/courses/Molbio/Protocols/ligation.html Ligation Protocol] | ||
| - | # [http://www.bio.davidson.edu/courses/Molbio/Protocols/Promegacompcells.pdf Transformation] [http://www.bio.davidson.edu/courses/Molbio/Protocols/transformation.html Short version] | + | # [http://www.bio.davidson.edu/courses/Molbio/Protocols/Promegacompcells.pdf Transformation] OR [http://www.bio.davidson.edu/courses/Molbio/Protocols/transformation.html Short version] |
# [http://www.bio.davidson.edu/courses/Molbio/Protocols/miniprepPrmega.html Promega miniprep] | # [http://www.bio.davidson.edu/courses/Molbio/Protocols/miniprepPrmega.html Promega miniprep] | ||
Revision as of 14:43, 31 May 2007
- What is the list of DNA parts that we will need for the first stage, second stage, entire project?
- ERIN and SABRIYA Which DNA parts exist in the registry?Answer1More Parts
- ERIN and SABRIYAWhich DNA parts will have to be designed? Will they be synthesized or produced by PCR?
[[loxP sites][ Hin/Hix CRE gene Degradation Tags
- [http://www.bio.davidson.edu/courses/Molbio/Protocols/reagents.html Common molecular reagents]
- [http://partsregistry.org/Assembly:Standard_assembly Standard Assembly]
- [http://www.bio.davidson.edu/courses/Molbio/Protocols/anneal_oligos.html Building dsDNA with Oligos]
- [http://www.bio.davidson.edu/courses/Molbio/Protocols/pcr.html Setting up PCR mixtures]
- [http://www.bio.davidson.edu/courses/Molbio/Protocols/magnesium.html PCR and Mg2+ concentration]
- [http://www.bio.davidson.edu/courses/Molbio/Protocols/pourgel.html Pouring an agarose gel]
- [http://www.bio.davidson.edu/courses/Molbio/Protocols/molwt.html Calculate MWs]
- [http://www.bio.davidson.edu/courses/Molbio/Protocols/digestion.html Digest DNA with restriction enzymes]
- Double Digest Guide
- [http://www.bio.davidson.edu/courses/Molbio/Protocols/clean_short.html Clean DNA (short protocol)]
- [http://www.bio.davidson.edu/courses/Molbio/Protocols/gels2002/1kbladder.pdf 1kb MW markers]
- [http://www.bio.davidson.edu/courses/Molbio/Protocols/SAP.html Shrimp Alkaline Phosphatase]
- [http://www.bio.davidson.edu/courses/Molbio/Protocols/Qiagen_gelpure.html Qiagen QIAquick Gel Purification]
- [http://www.bio.davidson.edu/courses/Molbio/Protocols/gelpure.html ElectroElute Gel Purification]
- [http://www.bio.davidson.edu/courses/Molbio/Protocols/ligation.html Ligation Protocol]
- [http://www.bio.davidson.edu/courses/Molbio/Protocols/Promegacompcells.pdf Transformation] OR [http://www.bio.davidson.edu/courses/Molbio/Protocols/transformation.html Short version]
- [http://www.bio.davidson.edu/courses/Molbio/Protocols/miniprepPrmega.html Promega miniprep]
