The What's and How's

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  • What is the list of DNA parts that we will need for the first stage, second stage, entire project?
  • ERIN and SABRIYA Which DNA parts exist in the registry?Answer1More Parts
  • ERIN and SABRIYAWhich DNA parts will have to be designed? Will they be synthesized or produced by PCR?

loxP sites Hin/Hix CRE gene Degradation Tags

  • Which plasmids will be used from the registry?
  • Where would biobricks be located?
  • MALCOLM What is the protocol for assembly for the first stage (restriction digestions, ligations, transformations)?
  1. http://www.bio.davidson.edu/courses/Molbio/Protocols/reagents.html Common molecular reagents
  2. http://www.bio.davidson.edu/courses/Molbio/Protocols/anneal_oligos.html Building dsDNA with Oligos
  3. http://www.bio.davidson.edu/courses/Molbio/Protocols/pcr.html Setting up PCR mixtures
  4. [[http://www.bio.davidson.edu/courses/Molbio/Protocols/magnesium.html PCR and Mg2+ concentration]]
  5. http://www.bio.davidson.edu/courses/Molbio/Protocols/pourgel.html Pouring an agarose gel
  6. http://www.bio.davidson.edu/courses/Molbio/Protocols/molwt.html Calculate MWs
  7. http://www.bio.davidson.edu/courses/Molbio/Protocols/digestion.html Digest DNA with restriction enzymes
  8. Double Digest Guide
  9. http://www.bio.davidson.edu/courses/Molbio/Protocols/clean_short.html Clean DNA (short protocol)
  10. http://www.bio.davidson.edu/courses/Molbio/Protocols/gels2002/1kbladder.pdf 1kb MW markers
  11. http://www.bio.davidson.edu/courses/Molbio/Protocols/SAP.html Shrimp Alkaline Phosphatase
  12. http://www.bio.davidson.edu/courses/Molbio/Protocols/Qiagen_gelpure.html Qiagen QIAquick Gel Purification
  13. http://www.bio.davidson.edu/courses/Molbio/Protocols/gelpure.html ElectroElute Gel Purification
  14. http://www.bio.davidson.edu/courses/Molbio/Protocols/ligation.html Ligation Protocol
  15. http://www.bio.davidson.edu/courses/Molbio/Protocols/Promegacompcells.pdf Transformation http://www.bio.davidson.edu/courses/Molbio/Protocols/transformation.html Short version
  16. http://www.bio.davidson.edu/courses/Molbio/Protocols/miniprepPrmega.html Promega miniprep
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