Tokyo Alliance: Design/Method

From 2006.igem.org

Top : Introduction : Significance : Design/Method : Results/Conclusion : About Tokyo Alliance

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Contents 1 Systematic design concept/method 1.1 Abstraction Phase 1.1.1 State Transition Diagram 1.1.2 Logic Gates 1.1.3 Block Diagram (System/Device) 1.2 Construction Phase 1.2.1 Up/Mid/Downstream 1.2.2 working model 1.2.3 DNA Construction Procedure

Systematic design concept/method

Abstraction Phase

State Transition Diagram

• Combination of Noughts-and-Crosses
  • 362,880(=9!)[patterns] (sum up all patterns)
  • 255,168 (As a game)
  • 26,830 (Eliminating symmetrical pairs)

We added the following rules to reduce the number of transition functions and inputs.

1st 

SYANAC goes to the center of the board. (sq#5)

2nd

Human goes to the specific corner(sq#1) or the edge(sq#4) of the board.

3rd

SYANAC must choose sq#3

Therefore, SYANAC's conbination patterns and inputs are as follows:

• SYANAC
  • 25 combination patterns(Eliminating symmetrical pairs)
  • 7 types of inputs

The State Transition Diagram shown right describes SYANAC's behavior based on this rule.

Logic Gates

Here we show you the logic gates of each sq# according to the state transition.

Block Diagram (System/Device)

Construction Phase

Up/Mid/Downstream

working model

• AND-AND gate (Repressor+Repressor)

• AND-AND gate (Activator+Repressor)

DNA Construction Procedure

• Midstream Parts Insertion

[http://131.112.128.86/downloads/tokyo_mid_insertion.mov Midstream Parts Insertion Procedure (.mov)] (Requires QuickTime)