From 2006.igem.org

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Summary of the meeting on 2005.08.22, Monday

Participants

• Birgit Dreier (Biologist that works with Zincfingers in Eucariots)
• Jonas Nart
• Tamara Ulrich
• Simon Barkow
• Alexander Roth
• Herve Vanderschuren
• Robin Künzler

Results

• We learned that it is possible to create a Zincfinger protein for any DNA-Sequence. But to be fast, we better chose sites that alredy exist and have alredy been tested.
• The longer the Zincfingers are (3, 6, 9 ...), the better is their specificity.
• The papers (noted by Simon) tell exactly how to build zincfingers.
• We should put the binding site into the promoter region itself. The best thing would be to put it between the promoter and coding region. We should use multiple binding sites for our zincfinger.
• Doing some tests would be very useful.
• Degradation: Ms. Dreier "thinks", that zincfingers are quite stable.
• Ms. Dreier didn't work with bacterial cells like e-coli. She says that we could possibly find some research on it.
• We learned that (in eucariotes) chances are slim to repress if we only use the zincfinger for blocking. Ms. Dreier thinks that a repressor attached to to zincfinger is required. She says that activation is usually dominant.
• So the zincfinger would be used to get the repressor to the right place.
• In general, she thinks that using zincfingers in our project is a good idea.