Aromatic Bacteria : Chiba 2006

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*9/12--We got a gene that codes geraniol synthase.  
*9/12--We got a gene that codes geraniol synthase.  
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*9/5--We solved a yesterday's problem. PUC-EBI was very dirty so it caused a lot of problem. After cleaning it, We got a clean band of PUC-EBI（linear）.     
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''view [[Aromatic Bacteria, Chiba 2006,: News|more]]''
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*9/4--We want to make PUC-EBIY. We got EBIY (it's orange color),but couldn't get PUC(vector).A band of PUC-EBI（linear）was very weak ,and we could see several bands. So we discussed what caused this problem. 
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*8/29--We discussed about the light sensor bacteria. 
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*8/12--Our first experiment using an aroma oil(Sweet Flavor-smells like orange).  10μl of oil was necessary for LB medium(solid and liquid) to smell good, or at least, better. 
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*8/4 -- We created parts J29021(generator-3 enzymes that is used to produce a lycopene) and J29022(device-light receptor and LacI).
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*8/4 -- We added EuY(J29030) and carene cyclase(J29036) to the Registry. 4 changes were made to carene cyclase to remove restriction sites of ecoR1 and pst1.
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Revision as of 04:11, 27 September 2006

Contents

concept

Aromaken4.jpg

Wow!! By lighting bacteria, a refreshing scent stars filling the room. In the dark, the light sensing E.coli will turn orenge. With the red light on, the color of E.coli fades out, and aroma is produced instead.

members

schedule

            9/21   22   23   24  25  26  27  28  29  30
Daisuke      x     x                                               
Kensuke                                   
Mayu                               
Shizuka                                                        
Keiichi


How it works

How it works.jpg


aroma

Firstly,we are going to make E.coli that smell good!

We are going to get gene that codes these products synthases.

[http://en.wikipedia.org/wiki/Carene Carene]
[http://en.wikipedia.org/wiki/Limonene Limonene]
[http://en.wikipedia.org/wiki/Pinene Pinene]
[http://en.wikipedia.org/wiki/Linalool Linalool]
[http://en.wikipedia.org/wiki/Geraniol Geraniol]
[http://en.wikipedia.org/wiki/Farnesene Farnesene]




light receptor

Secondary,We are going to make a light receptor that react different wavelength.

PCB reacts redlight (that is about 660nm). If we change PCB to PΦB and PEB , the absorption wavelength shifts 15nm respectively.

Light receptor.jpg

These are a structure of chromophore. Chromophore.jpg

carotenoid

papers

Aroma etc…

[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15236249 Yasuo Yoshikuni(2004)-Mono and diterpene production in Escherichia coli.]


[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=15577217&dopt=Abstract Gisho Honda(2004)cDNA isolation and functional expression of myrcene synthase from Perilla frutescens.]





Light receptor etc…

[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16458890&dopt=Abstract Takayuki Kohchi(2006)-Metabolic engineering to produce phytochromes with phytochromobilin, phycocyanobilin, or phycoerythrobilin chromophore in Escherichia coli.]

[http://www.pnas.org/cgi/content/full/101/50/17334?ck=nck J.Clark Lagarias(2004)-Harnessing phytochrome's glowing potential]


carotenoid etc…






news

  • 9/23--We succesed yesterday's experiment. The smell of geraniol that is produced E.coli was a kind of citrus.
  • 9/22--We tested efficency of E.coli that makes geraniol.
  • 9/21--We are trying to make E.coli that makes geraniol.
  • 9/12--We got a gene that codes geraniol synthase.


view more

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