Davidson Parts

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'''''Hin gene: Part BBa_J31000'''''
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[[Hin gene: Part BBa_J31000]]
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* Used Hin 100-1
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Primers for Hin gene the 72nd base was mutated from a "T" to a "C" in order to remove a naturally occuring SpeI site. The mutatation is in '''bold'''.
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[[Primers for Hin gene with LVA degredation tag]]
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*Used HinLVA-2
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'''Forward primer'''
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[[Recombinational Enahancer: Part BBa_J3101]]
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* used RE-11
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5’ gcattctagaatggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttac'''C'''agtgcaaattg 3’
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[[HixC]]
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*Used Hix C-2
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'''Reverse primer'''
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[[Resistance Genes]]
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*Tet Back- used TB1
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5’ atgcctgcaggcggccgcactagtttaattcattcgtttttttatac  3’
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[[Construction Intermediates]]
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[[Primers for Hin gene with LVA degredation tag]]
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[[Recombinational Enahancer: Part BBa_J3101]]
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Latest revision as of 16:11, 31 July 2006

Hin gene: Part BBa_J31000

  • Used Hin 100-1

Primers for Hin gene with LVA degredation tag

  • Used HinLVA-2

Recombinational Enahancer: Part BBa_J3101

  • used RE-11

HixC

  • Used Hix C-2

Resistance Genes

  • Tet Back- used TB1

Construction Intermediates

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