ETH2006 copy
From 2006.igem.org
(→Implementation alternatives) |
(→PoPs duplexer device) |
||
| Line 2: | Line 2: | ||
This device has two PoPs outputs which in terms of activity should be duplicates of the input. | This device has two PoPs outputs which in terms of activity should be duplicates of the input. | ||
| + | |||
| + | PoPS duplexers are needed if we want to preserve the strict PoPS input/output interface of devices. | ||
=== Implementation alternatives === | === Implementation alternatives === | ||
| Line 15: | Line 17: | ||
| align="center" colspan="3" | ''PoPS Duplexer Variants'' | | align="center" colspan="3" | ''PoPS Duplexer Variants'' | ||
|} | |} | ||
| + | |||
| + | ==== General Duplexer ==== | ||
| + | The general duplexer variant offers most flexibility since both oututs can be controlled seperately. Ribosome binding sites of different strengths can be used to have different amplification factors on the ouptuts - which can be important if subsequent devices require input signals of different strengths. Another working point are the promoters, different promoter strengths also affect output signal strength. | ||
| + | |||
| + | ==== Simplified Duplexer ==== | ||
| + | The simplified duplexer still facilitate specific output amplification, however, the only starting point is the promoters. Since both promoters are stimulated by the same activator, it might be more difficult to find appropriate promoters. | ||
| + | |||
| + | ==== Duplexing by Concatenation ==== | ||
| + | The simplest way of PoPS duplexing is simple concatenation of to devices. The | ||
=== Modeling === | === Modeling === | ||
Revision as of 18:11, 29 October 2006
Contents |
PoPs duplexer device
This device has two PoPs outputs which in terms of activity should be duplicates of the input.
PoPS duplexers are needed if we want to preserve the strict PoPS input/output interface of devices.
Implementation alternatives
| General Duplexer | Simplified Duplexer | Duplexing by Concatenation |
 |  |  |
| PoPS Duplexer Variants | ||
General Duplexer
The general duplexer variant offers most flexibility since both oututs can be controlled seperately. Ribosome binding sites of different strengths can be used to have different amplification factors on the ouptuts - which can be important if subsequent devices require input signals of different strengths. Another working point are the promoters, different promoter strengths also affect output signal strength.
Simplified Duplexer
The simplified duplexer still facilitate specific output amplification, however, the only starting point is the promoters. Since both promoters are stimulated by the same activator, it might be more difficult to find appropriate promoters.
Duplexing by Concatenation
The simplest way of PoPS duplexing is simple concatenation of to devices. The
