Answer T2


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  1. From what we have seen, once an invertasome is formed with two hix sites, recombination can occur multiple times.
  2. A new invertasome would have to form with new hix sites to cause a new recombination.
  3. Each time recombination occurs, two negative supercoils are used, a topological knot is produced, and the internal halves of two hix sites are exchanged.
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