Berkeley Work Schedule and Progress

From 2006.igem.org

Current Work:

• Conjugation testing:
  
  • oriT - Test plasmid mobility
    
  • TraJf, TraJr - does promoter induction cause conjugation to initiate?
    
  • Test mobilization construct
    
• Construction:
  
  • Spo0A - QuikChange (Stratagene kit site directed mutagenesis) out the Eco site and finish mobilizable construct for OriTf
• Lock and Key Strength
  • Flourescence assays
• Other
  • onRFP - Find out cause for onRFP sporadic behaviour during conjugation (tetR presence?)

Milestones:

November:
Measurement data for constructs, construction continues

September:
Project training continues, implementation begins

July-August:
Theoretical project design and background research on bacterial conjugation and technique training

May-June:
Synthetic Biology primer and project brainstorming