Brown:Summer workshops


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Location: Our new lab

Monday June 5, 2006 (10am-12pm)

Lab Safety

  • clothing/protection
  • common sense
  • waste
  • storage

Overview of research basics

  • lab notebook
  • resources
  • why am I doing this experiment?

Making stock solutions

  • pipetting
  • weighing
  • graduated cylinders
  • volumetric flasks
  • % solutions (vol/vol, g/vol)
  • molar vs. normal solutions
  • buffers

HOMEWORK!!! (this will make my workshop shorter)

  • 1. You need to make 3% BSA in 10mM Acedic Acid. Do all the necessary calculations. (If you don't know where to start, don't be discouraged - we'll show you on Monday June 5th).
  • 2. You need to make 2 liters of the following solution (all are liquids except for the salt): 10% glycerol, 0.9% NaCl, 0.01% Tween 20, 0.01% Tween 80. Show calculations.

Led by: Ana (10am-12pm)
Lab safety training with engineering group from 1pm - 5pm Please email Karen to join group. All students must have completed lab safety training to work in the lab.

Tuesday June 6, 2006 (10am-12pm)

Restriction digest
Gel electrophoresis
Led by: Azeem and Jason

Thursday June 8, 2006 (1:00pm - 2:30pm)

A tentative list of topics:
1. bacterial strains
2. how to grow bacteria (solid/liquid culture)
3. how to avoid contamination
4. how to make competent cells
5. how to grow plasmids within bacteria
6. antibiotics resistance
7. blue/white selection
8. iGEM specific information
Led by: Jamie Gagnon

Friday June 9, 2006 (10am-12pm)

Led by: Azeem and Jason

Personal tools
Past/present/future years