JCA121504-Construction of pAC-LuxInv
From 2006.igem.org
PCR 837F/R on pSupDlux (1743 bp, NotI/BamHI) Sub into pAC-TetInv (NotI/BamHI) Product is pAC-LuxInv, bp
ca837F Forward NotI oligo for lux GGTATGCGGCCGCTTAATTTTTAAAGTATGGGCAATC ca837R Reverse BamHI oligo for lux CACTTggatccGTAATGACAGATAATTTTACTC