Meeting Minutes for June 23, 2006

From 2006.igem.org

Matters Arising

No one filled out the calendar
We needed biohazard training – we got it

In the Lab

Some parts consistently not transforming, checked plates and cells are working
Restriction digests on promoters
Electroporation? – brute force, have set up in lab, electrocute cells, some live, opens cell membrane, worth a try – Meghan is interested, will investigate further
First need to make electrocompetent cells, can use DH5alpha
MIT sending plates?
Still need paperwork for magnetotactic bacteria – John
Should begin to culture them – Brendan

Magnetotactic Bacteria

Annie explains luciferase project
Wessel suggested using avadin protein
We have name of someone at Woodshole that may be working with them – Victoria and Annie
Would require a KO? Don’t see anything about it in paper
Can we get the plasmid from them? It’s in Japan, but would alleviate our problem
Don’t need the whole plasmid, just the backbone
Peter – looking into plasmid and vector backbone
Also uses transconjugation, which we have no experience with

Bacterial Maze

Might not work because we need a double KO, which would take too long
We have the MotB KO
Try to use that strain to do something else
Bacterial communication to induce or inhibit motility – Bacterial Freeze Tag
“It” bacteria send out AHL
one plasmid in receiver has LuxR attached to LacI
other would have promoter attached to MotB attached to cI then PcI then GFP then LacI (which represses first promoter) cI inhibits PcI
IPTG inhibits LacI inhibition
Basal level of IPTG allows for motility, repression gets no GFP
How close does the AHL have to be to trigger the other cell? Promoter sensitivity?
Contact Tripathi (fluidics chamber)

Go back to design phase and come up with something? Simpler?
Should look at the parts available and document what they do, a chart perhaps
Look at the basics of the parts, two or three parts together
Google spread sheet?
Meet at Barus and Holley tomorrow @ noon

Poster Session Aug 3rd – Brendan