Seeding (for Induction)
From 2006.igem.org
1. Prepare desired volume of 1X LB all in one culture tube (calculate 1mL per seeding).
2. Add desired antibiotic (1uL per mL for Ampicilin).
3. Split the total volume by pipetting 1mL into additional culture tubes until each tube has 1mL.
4. Pick colonies and pipet up and down in LB to mix thoroughly.
5. Place in S-I overnight.
NB: Close tubes only partially to let air in.
