Timeline

From 2006.igem.org

[http://2006.igem.org/Minutes Minutes]

Week 10: 21^th - 25^th August

1. 22nd-23rd: Meeting with Cambridge team
2. Finish presentation

For the lab:

1. Get a working arsenic sensor. Obviously.
2. Have the constructs of arsR and lambda cI complete.
3. Site directed mutagenesis on the urease gene with the aim of making it into a biobrick
4. Possible construction of the hybrid promoter, if primers have arrived.

Week 6: 24^th - 28^th July

1. 24th-25th: Meeting with the Cambridge and Imperial teams at Cambridge.

Week 5: 17^th - 21^st July

1. Cambridge presentation meeting thursday. The output from this will be a good layout for the presentation, which can be improved on friday.
2. PCR primers for Urease part ordered
3. Website host adress (www.macteria.co.uk) acquired
4. Batch of 50 badges made (so we can take them to cambridge)
5. "Turtle" characterised (if it arrives)
6. Investigate available growth mediums in preparation for further experimentation next week
7. Minipreps, digests and gels for cells transformed with (hopefully) biobricked arsenic and lacZ parts.
8. Joining of arsenic and lacZ parts, with a terminator, and transforming into E. coli

Week 4: 10^th - 14^th July

1. Locate parts in the registry for the modelling of the arsenic biosensor and the 3D structure builder
2. Continuing in the lab with putting the arsR and new lacZ genes into biobrick constructs and trying to solve issues with isolating DNA fragments from gels
3. Devising new pH experiments to calibrate the sensor
4. Badge making, postcard making, locating possible people to send them to
5. Organising Cambridge trip
6. Initial modelling of the biosensor, finding out all the reactions involved and turning them into equations
7. Set up new website

Week 3: 3^rd - 7^th July

Objectives for the arsenic sensor:

Assess the effects of the:

1) bacterial population density on pH

2) growth phase on pH change

3) concentration of lactose on pH

4) time on pH

All effects are to be measured on the pH of the liquid cultures with the Lacz deficient strain of E-coli.

Due to problems in the lab, the above objectives are revised to next week. These problems are illustrated in the [http://2006.igem.org/Lab_Work Lab work section]. Objectives for this week are now to get the first PCR primers made to assemble our first biobricks. Tests for the production of lactic acid on L-agar plates using the LacZ plasmids should be started tomorrow afternoon after the lecture from Dr Elfick.

Objectives for the 3d structure builder:

1) Determine whether to use the Urease or Phosphatase enzyme to precipitate a solid substrate

Week 2: 26^th - 30^th June

More Brainstorming

Week 1: 19^th - 23^rd June

Brainstorming

[http://2006.igem.org/University_of_Edinburgh_2006 Main page]