BU06:Team A

{{BU06}}
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|<font size="4"> '''Team Goal: Photography System Assembly and Testing''' </font>
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back to the [[:Category: Boston University 2006|BU iGEM main page]]
[[Category: Boston University 2006| Team A]]
== '''Members''' ==
{| width="600"
|- style="background:#E1E1E1;" 
| <font face="century gothic">'''Mac Cowell'''
| <font face="century gothic">[mailto:macowell@bu.edu macowell@bu.edu]    
| <font face="century gothic"> aim: cxmac2
|-
| <font face="century gothic">'''Christine Woods'''
| <font face="century gothic">[mailto:cwoods@bu.edu cwoods@bu.edu]
| <font face="century gothic">aim: xoptopinope
|
|- style="background:#E1E1E1;" 
| <font face="century gothic">'''Nancy Mendonca Davis'''
| <font face="century gothic">[mailto:nancymd@bu.edu nancymd@bu.edu]
| <font face="century gothic"> 
|-
| <font face="century gothic">'''Nadav Ivzan'''
| <font face="century gothic">[mailto:nivzan@bu.edu nivzan@bu.edu]
| <font face="century gothic">aim:kingos82 msn:kingos19@hotmail.com
|}

== Meeting Schedule ==

Next meeting:

== Current Progress ==
===Photosystem parts===

''Light Sensing Inhibitor:'' 
* [http://partsregistry.org/Part:BBa_I15008 BBa_I15008] (well: 21A  plate: iGEM2006 DNA-2) 
* [http://partsregistry.org/Part:BBa_I15009 BBa_I15009] (well: 21C  plate: iGEM2006 DNA-2)
* [http://partsregistry.org/Part:BBa_I15010 BBa_I15010] (Note: Must be used in E.coli deficient in natural EnvZ.  Also, not in 2006 iGEM dna distribution)
* [http://partsregistry.org/Part:BBa_R0082 BBa_R0082] (well: 15P  plate: iGEM2006 DNA-1)

'''[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2006partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2006&group=BU BU's temporary parts]'''

===Assembly of parts===
* [[Biobrick delivery]] - overview of the the 2006 iGEM DNA distribution plates.

* [http://partsregistry.org/Help:How_to_make_agar Color-codes] that indicate the corresponding biobrick's antibiotic resistance. (Each brick has a certain antibiotic resistance.  Biobricks stored in Plate 1 are all Ampicilin resistant.  Those in Plate 2 have a color-code).

* [http://openwetware.org/wiki/BioBricks_construction_tutorial biobrick construction tutoral] and related protocols.
** [http://openwetware.org/wiki/Silver:_BB_Strategy Summary] of biobrick construction from the Silver lab.
** [http://openwetware.org/wiki/Synthetic_Biology:BioBricks/3A_assembly 3A assembly] is an interesting alternative that avoids the gel purification step.

==Questions==

* What strain of e. coli should we use?  How do we make those cells competent?  

* How do we find out which antibiotic a particular DNA on Plate 2 is resistant to?  The [[Biobrick delivery|Biobrick Delivery instructions]] say that we should use the [http://partsregistry.org/cgi/assembly/libraries.cgi?id=6 Registry Repository] to find out, but it doens't seem to list each part's antibiotic resistance on there.
** The bottom of each well is [http://partsregistry.org/Help:How_to_make_agar color-coded] to indicate the antibiotic resistance.

* How do we obtain part I15010?
**  Email Meagan at the registry (meaganl AT mit DOT edu) and copy James Brown (jrb62 AT cam.ac.uk)

* How do we obtain EnvZ- e. coli?
**  Ask frank - he seems to know.  

* Who knows what we can buy now and what general lab supplies are available for our use?

==Protocols==


== References ==
* [http://openwetware.org/wiki/Synthetic_Biology:BioBricks Synthetic_Biology:BioBricks] TONS of overlooked info & protocols on OWW.
* The [http://partsregistry.org/cgi/assembly/libraries.cgi index] of the 384-well plates.
* The [http://partsregistry.org/Featured_Parts:Light_Sensor Light Sensing System].
* [http://openwetware.org/wiki/Protocols Protocols]
* [http://partsregistry.org/Part:BBa_M30109 Reference Part M30109]
* Bacterial Photography [http://openwetware.org/wiki/BE.109:Systems_engineering/Basic_bacterial_photography:_black_and_white Lab] w/M30109 part.
* [http://www.hamline.edu/depts/biology/courses/biocon2/luxback.html Background] on the lux operon
* [http://encyclopedia.laborlawtalk.com/bioluminescence Bioluminescence. Interesting.]