Brown:Equipment needed

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{| border=1 cellspacing=0 cellpadding=5
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{| border=1 cellspacing=0 cellpadding=1
|-  
|-  
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| type/size
| type/size
| quantity
| quantity
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| Status
+
| Have(x)
 +
| Location
 +
| Comments
|-
|-
| '''Equipment'''
| '''Equipment'''
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| -80°C
| -80°C
| 1
| 1
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| we have space in a -80
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| JWW basement
|-  
|-  
|  
|  
| -20°C
| -20°C
| 1
| 1
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| yes
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| JWW228
|-  
|-  
|  
|  
| 4°C
| 4°C
| 1
| 1
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| yes
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| JWW228
|-  
|-  
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| incubator  
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| incubator (what kind?)
|  
|  
| 1
| 1
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| might work (heh)
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| JWW228
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|-
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| pH meter
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|
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| 1
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|
|-  
|-  
| hot bath
| hot bath
|  
|  
| 1
| 1
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| we have access
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| JWW218
|-  
|-  
| cold bath
| cold bath
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| autoclave
| autoclave
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|  
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|
| 1
| 1
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| we have access
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|JWW 4th Floor
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|
 +
|-  
|-  
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| cell shaker/incubator
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| cell shaker inside 37 degree incubator
|  
|  
| 1
| 1
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| we have acess
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| JWW 3rd floor
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|-  
| source of ddH2O
| source of ddH2O
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|-  
| microcentrifuge (w/temp cntrl.)
| microcentrifuge (w/temp cntrl.)
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|  
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| temp. cntrl not essential
| 1
| 1
|-  
|-  
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| 50mL and 15mL
| 50mL and 15mL
| 1
| 1
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| yes - 15 mL
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| very old, JWW228
|-  
|-  
| Electrophoresis gel set
| Electrophoresis gel set
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| 1
| 1
|-  
|-  
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| Manual pipettors / bulbs
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|
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| 2
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|-
| bunsen burner
| bunsen burner
|  
|  
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| 2
| 2
|-  
|-  
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| pasteur pipettes
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|
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| 1 box
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|-
| '''Gloves (boxes)'''
| '''Gloves (boxes)'''
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| centrifuge tubes
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| eppendorf tubes
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|  
|  
|  
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|  
|  
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| 200μL (PCR)
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| 200μL (thin wall for PCR)
| 1000
| 1000
|-  
|-  
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|
|
|
|
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|-
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| pH standard buffers for meter
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|
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| 1
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|-
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| 70% ethanol
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|
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|
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|-
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| anitibiotics
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|
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|
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|-
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|
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| ampicillin
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|
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|-
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|
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| kanamycin
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|
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|-
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|
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| puromycin
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|
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|-
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| Agarose
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|
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|
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|-
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| Ethidium Bromide
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|
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|
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|-
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| tris[hydroxymethyl]aminomethane
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|
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|
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|-
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| Acetate
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|
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|
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|-
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| EDTA
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|
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|
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|-
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| Gel Loading Dye
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|
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|
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|-
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| DNA ladder
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|
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|
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|-
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| Restriction enzymes and Buffers
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|
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|
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|-
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|
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| EcoRI
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|
 +
|-
 +
|
 +
| SpeI
 +
|
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|-
 +
|
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| XbaI
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|
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|-
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|
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| PstI
 +
|
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|-
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| Taq Polymerase (Master Mix)
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|
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|
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|-
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| 100% ethanol
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|
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|
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|-
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| Socium Acetate
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|
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|
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|-
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| NaCl
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|
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|
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|-
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| T4 DNA ligase
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|
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|
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|-
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| T4 DNA ligase Buffer
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|
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|
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|-
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| liquid LB Broth
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|
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|
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|-
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| LB with agar
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|
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|
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|-
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| TSS Buffer
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|
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|
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|-
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| Mini-Prep Kit
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|
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|
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|-
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| Maxi-Prep Kit
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|
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|-  
|-  
|}
|}

Latest revision as of 16:50, 19 June 2006

type/size quantity Have(x) Location Comments
Equipment
freezers/fridges
-80°C 1 we have space in a -80 JWW basement
-20°C 1 yes JWW228
4°C 1 yes JWW228
incubator (what kind?) 1 might work (heh) JWW228
pH meter 1
hot bath 1 we have access JWW218
cold bath 1
autoclave 1 we have access JWW 4th Floor
cell shaker inside 37 degree incubator 1 we have acess JWW 3rd floor
source of ddH2O 1
hot plates w/ stirring 2
stir bars (set) 1
PCR thermocycler 1
microcentrifuge (w/temp cntrl.) temp. cntrl not essential 1
centrifuge 50mL and 15mL 1 yes - 15 mL very old, JWW228
Electrophoresis gel set 4
Gel Power source 1
UV Gel viewer 1
UV Gel camera 1
Pipettemen
20μL 4
200μL 4
1000μL 4
Electronic pipettor can use 1-25mL pipettes 1
Manual pipettors / bulbs 2
bunsen burner 2
sparkers 2
wire loop to pick a bac clone 3
metal loop to spread bacteria 3
Glassware
Bottles
1L 4
500mL 8
100mL 8
Beakers
1L 2
250mL 8
Centrifuge tubes
50mL 100
15mL 100
Graduated cylinders

500mL

2
100mL 2
Electronic pipette tips (packages)
1mL 2
5mL 2
10mL 2
25mL 2
pasteur pipettes 1 box
Gloves (boxes)
small 1
medium 4
large 4
Plasticware
eppendorf tubes
1.5mL 2000
200μL (thin wall for PCR) 1000
Pipette tips
20μL a lot
200μL a lot
1000μL a lot
Bacterial cell culture plates 200
bacterial cell culture tubes (polypropylene) 100
tube racks/holders
for 1.5mL tubes 4
for 200μL tubes 2
Reagents
pH standard buffers for meter 1
70% ethanol
anitibiotics
ampicillin
kanamycin
puromycin
Agarose
Ethidium Bromide
tris[hydroxymethyl]aminomethane
Acetate
EDTA
Gel Loading Dye
DNA ladder
Restriction enzymes and Buffers
EcoRI
SpeI
XbaI
PstI
Taq Polymerase (Master Mix)
100% ethanol
Socium Acetate
NaCl
T4 DNA ligase
T4 DNA ligase Buffer
liquid LB Broth
LB with agar
TSS Buffer
Mini-Prep Kit
Maxi-Prep Kit
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