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TetR promoted with LuxI downstream. LuxI is an enzyme that produces AHL and will produce the red fluorescent protein (RFP). The AHL produced is exported from the cell where it then forms a complex with the LuxR protein that is produced by the AHL sensor within the Receiver cell. | TetR promoted with LuxI downstream. LuxI is an enzyme that produces AHL and will produce the red fluorescent protein (RFP). The AHL produced is exported from the cell where it then forms a complex with the LuxR protein that is produced by the AHL sensor within the Receiver cell. | ||
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[[Image:SenderCell.png]] | [[Image:SenderCell.png]] | ||
Revision as of 17:39, 13 July 2006
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Bacterial Freeze Tag
Overview
Sender Cell
TetR promoted with LuxI downstream. LuxI is an enzyme that produces AHL and will produce the red fluorescent protein (RFP). The AHL produced is exported from the cell where it then forms a complex with the LuxR protein that is produced by the AHL sensor within the Receiver cell.
AHL Sensor
The AHL sensor is TetR promoted and forms the Lux R protien which then forms a complex with AHL. This LuxR and AHL complex then activates the pLUXR promoter. Downstream of the pLUXR promoter is the LACI protein. LacI inhibit the pLAC promoter on the "Freeze Machine".
'Freeze Machine'
A promoter that is regulated by LacI will promote the production of LasI, MotB, and cI. This will subsequently inhibit the production of CFP and LasR. In the presence of LacI, however, MotB, LasI, and cI will not be produced. CFP will therefore be produced along with LasR and LacI. This results in the "freezing" of the cell.
