JCA121504-Construction of pAC-LuxInv
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| - | + | PCR 837F/R on pSupDlux (1743 bp, NotI/BamHI) | |
Sub into pAC-TetInv (NotI/BamHI) | Sub into pAC-TetInv (NotI/BamHI) | ||
Product is pAC-LuxInv, bp | Product is pAC-LuxInv, bp | ||
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GGTATGCGGCCGCTTAATTTTTAAAGTATGGGCAATC | GGTATGCGGCCGCTTAATTTTTAAAGTATGGGCAATC | ||
ca837R Reverse BamHI oligo for lux | ca837R Reverse BamHI oligo for lux | ||
| - | CACTTggatccGTAATGACAGATAATTTTACTC | + | CACTTggatccGTAATGACAGATAATTTTACTC |
Latest revision as of 00:16, 27 May 2006
PCR 837F/R on pSupDlux (1743 bp, NotI/BamHI) Sub into pAC-TetInv (NotI/BamHI) Product is pAC-LuxInv, bp
ca837F Forward NotI oligo for lux GGTATGCGGCCGCTTAATTTTTAAAGTATGGGCAATC ca837R Reverse BamHI oligo for lux CACTTggatccGTAATGACAGATAATTTTACTC
