BU06:Research
From 2006.igem.org
(Difference between revisions)
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''Light Sensing Inihibitor:'' BBa_I15008, BBa_I15009, BBa_I15010, BBa_R0082 | ''Light Sensing Inihibitor:'' BBa_I15008, BBa_I15009, BBa_I15010, BBa_R0082 | ||
- | ''Light Emission Device:'' (from lux operon) THIS IS WHAT WE NEED TO DO. | + | ''Light Emission Device:'' (from lux [http://en.wikipedia.org/wiki/Operon operon]) THIS IS WHAT WE NEED TO DO. |
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Light Sensor Parts: http://partsregistry.org/Featured_Parts:Light_Sensor and paper : http://www.nature.com/nature/journal/v438/n7067/full/nature04405.html | Light Sensor Parts: http://partsregistry.org/Featured_Parts:Light_Sensor and paper : http://www.nature.com/nature/journal/v438/n7067/full/nature04405.html | ||
- | It seems to repress gene expression by having red light inhibit phosphorylation which would activate a promoter. We would replace the LacZ protein coding region with our lux operon. | + | It seems to repress gene expression by having red light inhibit phosphorylation which would activate a promoter. We would replace the LacZ protein coding region with our lux [http://en.wikipedia.org/wiki/Operon operon]. |
Revision as of 20:43, 9 June 2006
Making E. coli Glow
Preliminary Design: -Light Sensing Inhibitor- -> -Light emission device-
Light Sensing Inihibitor: BBa_I15008, BBa_I15009, BBa_I15010, BBa_R0082
Light Emission Device: (from lux [http://en.wikipedia.org/wiki/Operon operon]) THIS IS WHAT WE NEED TO DO.
Light Sensor Parts: http://partsregistry.org/Featured_Parts:Light_Sensor and paper : http://www.nature.com/nature/journal/v438/n7067/full/nature04405.html
It seems to repress gene expression by having red light inhibit phosphorylation which would activate a promoter. We would replace the LacZ protein coding region with our lux [http://en.wikipedia.org/wiki/Operon operon].