Timeline
From 2006.igem.org
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| + | [http://2006.igem.org/Minutes Minutes] | ||
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| + | ==<font color='red'>Week 10: 21<sup>th</sup> - 25<sup>th</sup> August</font>== | ||
| + | |||
| + | #22nd-23rd: Meeting with Cambridge team | ||
| + | #Finish presentation | ||
| + | |||
| + | For the lab: | ||
| + | #Get a working arsenic sensor. Obviously. | ||
| + | #Have the constructs of arsR and lambda cI complete. | ||
| + | #Site directed mutagenesis on the urease gene with the aim of making it into a biobrick | ||
| + | #Possible construction of the hybrid promoter, if primers have arrived. | ||
| + | |||
| + | ==<font color='red'>Week 6: 24<sup>th</sup> - 28<sup>th</sup> July</font>== | ||
| + | |||
| + | #24th-25th: Meeting with the Cambridge and Imperial teams at Cambridge. | ||
==<font color='red'>Week 5: 17<sup>th</sup> - 21<sup>st</sup> July</font>== | ==<font color='red'>Week 5: 17<sup>th</sup> - 21<sup>st</sup> July</font>== | ||
| Line 5: | Line 21: | ||
#PCR primers for Urease part ordered | #PCR primers for Urease part ordered | ||
#Website host adress (www.macteria.co.uk) acquired | #Website host adress (www.macteria.co.uk) acquired | ||
| - | #Batch of 50 badges made (so we can take them to | + | #Batch of 50 badges made (so we can take them to cambridge) |
#"Turtle" characterised (if it arrives) | #"Turtle" characterised (if it arrives) | ||
#Investigate available growth mediums in preparation for further experimentation next week | #Investigate available growth mediums in preparation for further experimentation next week | ||
| - | + | #Minipreps, digests and gels for cells transformed with (hopefully) biobricked arsenic and lacZ parts. | |
| - | + | #Joining of arsenic and lacZ parts, with a terminator, and transforming into E. coli | |
| - | + | ||
==<font color='red'>Week 4: 10<sup>th</sup> - 14<sup>th</sup> July</font>== | ==<font color='red'>Week 4: 10<sup>th</sup> - 14<sup>th</sup> July</font>== | ||
Latest revision as of 09:33, 25 October 2006
[http://2006.igem.org/Minutes Minutes]
Week 10: 21th - 25th August
- 22nd-23rd: Meeting with Cambridge team
- Finish presentation
For the lab:
- Get a working arsenic sensor. Obviously.
- Have the constructs of arsR and lambda cI complete.
- Site directed mutagenesis on the urease gene with the aim of making it into a biobrick
- Possible construction of the hybrid promoter, if primers have arrived.
Week 6: 24th - 28th July
- 24th-25th: Meeting with the Cambridge and Imperial teams at Cambridge.
Week 5: 17th - 21st July
- Cambridge presentation meeting thursday. The output from this will be a good layout for the presentation, which can be improved on friday.
- PCR primers for Urease part ordered
- Website host adress (www.macteria.co.uk) acquired
- Batch of 50 badges made (so we can take them to cambridge)
- "Turtle" characterised (if it arrives)
- Investigate available growth mediums in preparation for further experimentation next week
- Minipreps, digests and gels for cells transformed with (hopefully) biobricked arsenic and lacZ parts.
- Joining of arsenic and lacZ parts, with a terminator, and transforming into E. coli
Week 4: 10th - 14th July
- Locate parts in the registry for the modelling of the arsenic biosensor and the 3D structure builder
- Continuing in the lab with putting the arsR and new lacZ genes into biobrick constructs and trying to solve issues with isolating DNA fragments from gels
- Devising new pH experiments to calibrate the sensor
- Badge making, postcard making, locating possible people to send them to
- Organising Cambridge trip
- Initial modelling of the biosensor, finding out all the reactions involved and turning them into equations
- Set up new website
Week 3: 3rd - 7th July
Objectives for the arsenic sensor:
Assess the effects of the:
- 1) bacterial population density on pH
- 2) growth phase on pH change
- 3) concentration of lactose on pH
- 4) time on pH
All effects are to be measured on the pH of the liquid cultures with the Lacz deficient strain of E-coli.
Due to problems in the lab, the above objectives are revised to next week. These problems are illustrated in the [http://2006.igem.org/Lab_Work Lab work section]. Objectives for this week are now to get the first PCR primers made to assemble our first biobricks. Tests for the production of lactic acid on L-agar plates using the LacZ plasmids should be started tomorrow afternoon after the lecture from Dr Elfick.
Objectives for the 3d structure builder:
1) Determine whether to use the Urease or Phosphatase enzyme to precipitate a solid substrate
Week 2: 26th - 30th June
More Brainstorming
Week 1: 19th - 23rd June
Brainstorming
[http://2006.igem.org/University_of_Edinburgh_2006 Main page]
