Imperial College 2006

From 2006.igem.org

(Difference between revisions)

Latest revision as of 16:42, 10 November 2006

[http://openwetware.org/wiki/IGEM:IMPERIAL/2006 Full Documentation of the project on OpenWetWare] Presentation Poster

Project Summary

We have used the traditional engineering approach to build a stable and flexible molecular oscillator

Our original design relies on population dynamics and was inspired by the Lotka-Volterra predation model

Every step of the development cycle (Specifications, Design, Modelling, Implementation, Testing/Validation) has been fully documented on our OWW site

Achievements

Derivation of the complete dynamical model, describing the main biochemical reactions driving our oscillator
Full theoretical analysis and detailed computer simulations, validating our design with regard to our specifications
Successful building and characterization of functional parts, providing the building blocks for the final oscillator

Main Project

	Engineering a Molecular Predation Oscillator For building our biological oscillator, the engineering cycle below was followed. Click on each of the stages of the engineering cycle below in order to find out about the different stages & aspects of the project.
[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Full_System 1) Specifications: ] Stable and robust oscillations Controllable frequency/amplitude Generic and modular [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Full_System/Design 2) Design: ] Mimics predator-prey dynamics based on the Lotka-Volterra model [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Full_System/Modelling 3) Modelling: ] [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/Theoretical_Analyses Full theoretical analysis] Modelling of the full system [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Full_System/Implementation 4) Implementation: ] All parts designed and modelled 4 functional & 5 intermediate submitted to the Registry [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Full_System/TestingValidation 5) Testing/ Validation: ] Successful characterization of sub-components.		[http://www.openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Full_System http://openwetware.org/images/f/fd/IGEM_IMPERIAL_DevCycle_Specifications.png] Click on picture for more info
	[http://www.openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Full_System/TestingValidation http://openwetware.org/images/2/20/IGEM_IMPERIAL_DevCycle_Validation.png] Click on picture for more info		[http://www.openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Full_System/Design http://openwetware.org/images/c/ce/IGEM_IMPERIAL_DevCycle_Design.png] Click on picture for more info

	[http://www.openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Full_System/Implementation http://openwetware.org/images/0/04/IGEM_IMPERIAL_DevCycle_Implementation.png] Click on picture for more info		[http://www.openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Full_System/Modelling http://openwetware.org/images/5/5f/IGEM_IMPERIAL_DevCycle_Modelling.png] Click on picture for more info

Secondary Projects

[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/popsblocker PoPs Blocker]

[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Bio_elec_interface Biological to Electrical Interface]

As a method of controlling the activation of the positive-feedback loop in our predator-prey based oscillator, we successfully created this part, which can be used as a general Pops Blocker:

This part is placed downstream of a promoter and prevents any Pops from the promoter passing through this part
When an accompanying Cre Recombinase plasmid becomes activated, the enzyme produced will permanently cut a section of DNA from the plasmid containing this part
Only then, the polymerase can pass through this part and transcribe downstream genes.

We also worked on a Biosensor for measuring AHL concentrations in order to establish a Biological to Electrical Interface this summer.

Using an enzyme to hydrolize the lactone AHL would result in a local change in pH
Measuring the change in pH gives a measurement of how much AHL is present.

Our Contributions to the Registry

Part Logo	Description	Link to registry	Built	Tested	Characterized	Sequenced	Sent
Final Constructs	(!) Favorite Part
	Final Prey Cell (!)	J37015	YES	YES	Pending	YES	YES
	Cre/Lox Prey Control (!)	J37027	YES	YES	YES	YES	YES
Test Constructs
	Final Polycistronic Predator Cell Test Construct (!)	J37016	YES	YES	YES	YES	YES
	Predator Cell, pLux Transfer Function (two promoters)	J37020	YES	YES	NO	NO	YES
Intermediate Parts
	AHL induced LuxR generator (for predator cell)	J37019	YES	N/A	N/A	YES	YES
	RBS + LuxR	J37033	YES	N/A	N/A	YES	YES
	Prey Cell Intermediate	J37034	YES	N/A	N/A	YES	YES
	LuxR + GFP	J37032	YES	N/A	N/A	YES	YES

Our Open Documentation

300+ Pages of documentation on OWW, 100's of Photographs, Extensive Modelling, Lab Notebook and much more!

[http://openwetware.org/wiki/IGEM:IMPERIAL/2006 iGEM@imperial] [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser Oscillator project] [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/popsblocker PoPs Blocker project] [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Bio_elec_interface AHL Biosensor project]	[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/LabCalendar Lab Notebook] [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Protocols Protocols] [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Journal_Club Journal Club] [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Lecture_Notes Lecture Notes]	[http://openwetware.org/wiki/BioSysBio:abstracts/2007/iGEM2006_Imperial_College BioSysBio Conference, UK] [http://openwetware.org/wiki/IGEM:IMPERIAL/Methodology Description of our Methodology]	[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Calendar/2006-7-26 UK iGEM Teams Meeting] [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Photos Photos] [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Instructors_Feedbacks Feedbacks on our experience]

The Team and Acknowledgements

Undergrads [http://openwetware.org/wiki/User:Cys Christin Sander] [http://openwetware.org/wiki/User:da104 Deepti Aswani] [http://openwetware.org/wiki/User:Farah Farah Vohra] [http://openwetware.org/wiki/User:Baijiongjun Jiongjun Bai] [http://openwetware.org/wiki/User:Johnsy John Sy] [http://openwetware.org/wiki/User:JohnChattaway John Chattaway] [http://openwetware.org/wiki/User:Jonny Jonathan Wells] [http://openwetware.org/wiki/User:Tom Tom Hinson]	The Imperial College iGEM team and advisors on the terrace of the Biochemistry building	Advisors [http://www3.imperial.ac.uk/people/r.kitney Prof. Richard Kitney] [http://www3.imperial.ac.uk/people/p.freemont Prof. Paul Freemont] [http://www3.imperial.ac.uk/people/d.mann Dr. David Mann] Kirsten Jensen [http://openwetware.org/wiki/User:Vincent Vincent Rouilly] [http://openwetware.org/wiki/User:chuehloo Chueh-Loo Poh] [http://openwetware.org/wiki/User:SxE00 Matthieu Bultelle]	Funding European Commission Imperial College Deputy Rector's Fund Imperial College Faculty of Engineering Imperial College Faculty of Natural Sciences

Imperial College 2006

From 2006.igem.org

Latest revision as of 16:42, 10 November 2006

Main Project

Secondary Projects

Our Contributions to the Registry

Our Open Documentation

The Team and Acknowledgements

Undergrads

Advisors

Funding

Views

Personal tools

Navigation

The Registry

Past/present/future years

Wiki related

Search

Toolbox

@@ Line 1: / Line 1: @@
 __NOTOC__
-== Welcome to '''''Imperial College''''' iGEM team! ==
+__NOTOC__
+<center>[[Image:Banner.png|800px]]</center>
-== Imperial on OpenWetWare ==
-<big>'''[http://openwetware.org/wiki/IGEM:IMPERIAL/2006 OpenWetWare]'''</big>
-== Progress ==
+<center><font size="4">'''[http://openwetware.org/wiki/IGEM:IMPERIAL/2006 Full Documentation of the project on OpenWetWare]'''</font></center>
-[[image:Oscilator3.jpg|right|frame|Successful oscilator modelling]]
+<center><font size="3">'''[[Media:IGEM_Imperial_2006_presentation.pdf |Presentation ]]'''</font></center>
-===Oscillator based on Predator Prey dynamics===
+<center><font size="3">'''[[Media:IGEM_Imperial_2006_Poster.pdf |Poster]]'''</font></center>
-We are creating a molecular system which is able to show oscillations which can be described by the lotka-volatra equations. The molecular oscillations are powered by two distinct cell populations, one which drives the predator and a second driving the prey. '''This flexible design will allow us to alter the amplitude and wavelength''' of the oscillator by changing the relative population sizes. The system uses quorum sensing to create culture wide synchronised oscillations of N-acyl homoserine lactone. The system is working in models using realistic values and we are currently devising ways to test the bio-bricks to analyse how their performance affects our models. '''Looks very promising…'''
-===BioClock===
-We are also working on a BioClock. The clock utilises predictable pulse transmission through cells in order to generate a 24 hour clock. From our designs it will be possible to tell the time at any time of the day with good accuracy. Our design alows for easy modulation of the time period involved, allowing us to potentially generate clocks that can measure a week, month or even year. This leads to the possibility of engineering a BioCalender.
+{| border="0" width="100%" style="padding: 5px; background-color: #ffffff; border: 2px solid #2171B8;"
+|-
+|width="20%" style="background:#2171B8"|[[Image:LogoFinal2.png|210px|center]]
+|width="40%" style="background:#2171B8"| <font face="arial" font style="color: #FFFFFF" >
+<center><big><u>'''Project Summary'''</u></big></center>
-'''Update:''' Biologically descriptive model completed and working ''in silico''!
-===BioCD===
+*We have used the traditional engineering approach to build a stable and flexible molecular oscillator
+*Our original design relies on population dynamics and was inspired by the Lotka-Volterra predation model
+*Every step of the development cycle (Specifications, Design, Modelling, Implementation, Testing/Validation) has been fully documented on our OWW site</font>
+|width="40%" style="background:#2171B8" | <font face="arial" font style="color: #FFFFFF" >
-Do you have a secrets to hide? Ever thought of storing information on bacteria cultures that would only be visible for your eyes or for that of your beloved's?
-Well...we have!
-We are devising a method of burning information onto a BioCD. The information will revealed upon application of a specific stimulus and can be read out via biological/electrical interface.
-== Team Members ==
+<center><big><u>'''Achievements'''</u></big></center>
-*Christin Sander
+<br>
-*Deepti Aswani
+*Derivation of the complete dynamical model, describing the main biochemical reactions driving our oscillator
-*Farah Vohra
+*Full theoretical analysis and detailed computer simulations, validating our design with regard to our specifications
-*Jiongjun Bai
+*Successful building and characterization of functional parts, providing the building blocks for the final oscillator
-*John Sy
+<br>
-*John Chattaway
+<br>
-*Jonathan Wells
+<br>
-*Tom Hinson
+</font>
+|}
-== Instructors ==
-*Prof. Richard Kitney
-*Prof. Paul Freemont
+==Main Project==
-*Dr. David Mann
+{| border="0" width="100%" align="center" style="padding: 5px; background-color: #ffffff; border: 2px solid #2171B8;"
-*Vincent Rouilly
+| width="20%" style="background:#2171B8"| <center>[[Image:Oscillator Icon.PNG|100px|center]] </center>
-*Chueh-Loo Poh
+| style="background:#2171B8" colspan="3"| <font face="arial" font style="color: yellow"> <center><big>'''Engineering a Molecular Predation Oscillator'''</big><br>
+<font face="arial" font style="color: #ffffff">For building our biological oscillator, the engineering cycle below was followed.<br>
+'''<font style="color: orange">Click</font> on <font style="color: orange">each</font> of the stages of the <font style="color: orange">engineering cycle</font> below in order to find out about the different stages & aspects of the project. '''</font></center>
+|-
+| style="background:#2171B8" width="200pt" rowspan="4" |
+[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Full_System <font face="arial" font style="color: yellow"> '''1) Specifications: '''</font>]
+<font face="arial" font style="color: #ffffff">
+*Stable and robust oscillations
+*Controllable frequency/amplitude
+*Generic and modular</font>
+[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Full_System/Design <font face="arial" font style="color: yellow">'''2) Design: '''</font>]
+<font face="arial" font style="color: #ffffff">
+*Mimics predator-prey dynamics based on the Lotka-Volterra model
+</font>
+[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Full_System/Modelling <font face="arial" font style="color: yellow">'''3) Modelling: '''</font>]
+<font face="arial" font style="color: #ffffff">
+*[http://www.openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/Theoretical_Analyses <font face="arial" font style="color: #FFFFFF">Full theoretical analysis</font>]
+*Modelling of the full system
+</font>
+[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Full_System/Implementation <font face="arial" font style="color: yellow"> '''4) Implementation: '''</font>]
+<font face="arial" font style="color: #ffffff">
+*All parts designed and modelled
+*4 functional & 5 intermediate submitted to the Registry
+</font>
+[http://www.openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Full_System/TestingValidation <font face="arial" font style="color: yellow">'''5) Testing/ Validation: '''</font>]
+<font face="arial" font style="color: #ffffff">
+*Successful characterization of sub-components.
+</font>
+| align="right"|[[Image:ArrowCurvedRight.png|110px]]
+| align="center" |[http://www.openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Full_System http://openwetware.org/images/f/fd/IGEM_IMPERIAL_DevCycle_Specifications.png]<font style="color:red"><br>Click on picture for more info</font>
+| align="left"|[[Image:ArrowCurvedDown.PNG|120px]]
+|-
+| align="center"|[http://www.openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Full_System/TestingValidation http://openwetware.org/images/2/20/IGEM_IMPERIAL_DevCycle_Validation.png]<font style="color:red"><br>Click on picture for more info</font>
+| align="center" rowspan="2"|[[Image:IGEM_IMPERIAL_DevCycle_FullSystem.png |206px|center]]
+| align="center"|[http://www.openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Full_System/Design http://openwetware.org/images/c/ce/IGEM_IMPERIAL_DevCycle_Design.png]<font style="color:red"><br>Click on picture for more info</font>
+|-
+| align="center"|[[Image:ArrowUp.png|20px]]
+| align="center"|[[Image:ArrowDown.png|20px]]
+|-
+| align="center"|[http://www.openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Full_System/Implementation http://openwetware.org/images/0/04/IGEM_IMPERIAL_DevCycle_Implementation.png]<font style="color:red"><br>Click on picture for more info</font>
+| align="center"|[[Image:ArrowLeft.png|150px]]
+| align="center"|[http://www.openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Full_System/Modelling http://openwetware.org/images/5/5f/IGEM_IMPERIAL_DevCycle_Modelling.png]<font style="color:red"><br>Click on picture for more info</font>
+|}
+==Secondary Projects==
+{| border="0"  width="100%" style="padding: 5px; background-color: #ffffff; border: 2px solid #2171B8;"  width="100%"
+|-
+!  width="50%" | <big>'''[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/popsblocker <font face="arial" font style="color: #2171B8">PoPs Blocker</font>]  '''</big>!! width="50%"| <big>'''[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Bio_elec_interface <font face="arial" font style="color: #2171B8">Biological to Electrical Interface]</font>'''</big>
+|-
+| style="background:#2171B8" width="50%" |
+<font face="arial" font style="color: #FFFFFF"> As a method of controlling the activation of the positive-feedback loop in our predator-prey based oscillator, we successfully created this part, which can be used as a general Pops Blocker:
+*This part is placed downstream of a promoter and prevents any Pops from the promoter passing through this part
+*When an accompanying Cre Recombinase plasmid becomes activated, the enzyme produced will permanently cut a section of DNA from the plasmid containing this part
+*Only then, the polymerase can pass through this part and transcribe downstream genes.
+</font>
+| style="background:#2171B8" width="50%" |
+<font face="arial" font style="color: #FFFFFF">We also worked on a Biosensor for measuring AHL concentrations in order to establish a Biological to Electrical Interface this summer.
+*Using an enzyme to hydrolize the lactone AHL would result in a local change in pH
+*Measuring the change in pH gives a measurement of how much AHL is present.
+<br>
+<br>
+<br>
+<br>
+</font>
+|}
+==Our Contributions to the Registry==
+{|border="0" width="100%" style="padding: 5px; background-color: #ffffff; border: 2px solid #2171B8;"
+! style="background:#2171B8" | <font face="arial" font style="color: #FFFFFF">Part Logo !! style="background:#2171B8" |<font face="arial" font style="color: #FFFFFF">Description</font> !! style="background:#2171B8" |<font face="arial" font style="color: #FFFFFF">Link to registry</font> !! style="background:#2171B8" |<font face="arial" font style="color: #FFFFFF">Built</font> !! style="background:#2171B8" |<font face="arial" font style="color: #FFFFFF">Tested </font>!! style="background:#2171B8" |<font face="arial" font style="color: #FFFFFF">Characterized </font>!! style="background:#2171B8" |<font face="arial" font style="color: #FFFFFF">Sequenced</font> !! style="background:#2171B8" |<font face="arial" font style="color: #FFFFFF">Sent</font>
+|-
+!style="background:#008080" |<font face="arial" font style="color: #FFFFFF">Final Constructs</font>
+!style="background:#008080"|<font face="arial" font style="color: orange">(!) Favorite Part</font>
+!style="background:#008080"|
+!style="background:#008080"|
+!style="background:#008080"|
+!style="background:#008080"|
+!style="background:#008080"|
+!style="background:#008080"|
+|-
+![[Image:J37015.PNG|250px]]!! Final Prey Cell <font face="arial" font style="color: orange">(!) </font> !! <bbpart>J37015</bbpart> !! YES !! YES !! Pending !! YES !! YES
+|-
+! [[Image:J37027.png|120px]] !! Cre/Lox Prey Control <font face="arial" font style="color: orange">(!) </font>!! <bbpart>J37027</bbpart> !! YES !! YES !! YES !! YES !!YES
+|-
+!style="background:#008080" |<font face="arial" font style="color: #FFFFFF">Test Constructs</font>
+!style="background:#008080"|
+!style="background:#008080"|
+!style="background:#008080"|
+!style="background:#008080"|
+!style="background:#008080"|
+!style="background:#008080"|
+!style="background:#008080"|
+|-
+![[Image:J37016.PNG|150px]]!! Final Polycistronic Predator Cell Test Construct <font face="arial" font style="color: orange">(!) </font>!! <bbpart>J37016</bbpart> !! YES !! YES !! YES !! YES!! YES
+|-
+! [[Image:J37020.png|170px]] !! Predator Cell, pLux Transfer Function (two promoters)  !! <bbpart>J37020</bbpart> !! YES !! YES !! NO !! NO !! YES
+|-
+!style="background:#008080" |<font face="arial" font style="color: #FFFFFF">Intermediate Parts</font>
+!style="background:#008080"|
+!style="background:#008080"|
+!style="background:#008080"|
+!style="background:#008080"|
+!style="background:#008080"|
+!style="background:#008080"|
+!style="background:#008080"|
+|-
+! [[Image:J37019.PNG|80px]] !! AHL induced LuxR generator (for predator cell) !! <bbpart>J37019</bbpart> !! YES !! N/A !! N/A!! YES !!YES
+|-
+! [[Image:J37033.PNG|65px]] !! RBS + LuxR !! <bbpart>J37033</bbpart> !! YES !! N/A !! N/A!! YES !!YES
+|-
+! [[Image:J37034.PNG|90px]] !! Prey Cell Intermediate!! <bbpart>J37034</bbpart> !! YES !! N/A !! N/A!! YES !!YES
+|-
+! [[Image:J37032.png|90px]] !! LuxR + GFP !! <bbpart>J37032</bbpart> !! YES !! N/A !! N/A!! YES !!YES
+|}
+==Our Open Documentation==
+*'''<font face="arial" font style="color: #2171B8">300+ Pages of documentation on OWW, 100's of Photographs, Extensive Modelling, Lab Notebook and much more!</font>'''
+__NOTOC__
+{| class="wikitable" border="0" cellpadding="10" cellspacing="1" style="padding: 5px; background-color: #ffffff; border: 2px solid #2171B8;text-align:center"
+!width="20%" align="left" valign="top" style="background:#2171B8; color:black"|
+*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006 <font face="arial" font style="color: #FFFFFF">iGEM@imperial</font>]
+*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser <font face="arial" font style="color: #FFFFFF">Oscillator project</font>]
+*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/popsblocker <font face="arial" font style="color: #FFFFFF">PoPs Blocker project</font>]
+*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Bio_elec_interface <font face="arial" font style="color: #FFFFFF">AHL Biosensor project</font>]
+!width="20%" align="left" valign="top" style="background:#2171B8; color:black"|
+*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/LabCalendar <font face="arial" font style="color: #FFFFFF">Lab Notebook</font>]
+*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Protocols <font face="arial" font style="color: #FFFFFF">Protocols</font>]
+*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Journal_Club<font face="arial" font style="color: #FFFFFF"> Journal Club</font>]<br\>
+*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Lecture_Notes <font face="arial" font style="color: #FFFFFF">Lecture Notes</font>]<br\>
+!width="20%" align="left" valign="top" style="background:#2171B8; color:black"|
+*[http://openwetware.org/wiki/BioSysBio:abstracts/2007/iGEM2006_Imperial_College <font face="arial" font style="color: #FFFFFF">BioSysBio Conference, UK</font>]
+*[http://openwetware.org/wiki/IGEM:IMPERIAL/Methodology <font face="arial" font style="color: #FFFFFF">Description of our Methodology</font>]
+!width="20%" align="left" valign="top" style="background:#2171B8; color:black"|
+*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Calendar/2006-7-26 <font face="arial" font style="color: #FFFFFF">UK iGEM Teams Meeting</font>]
+*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Photos <font face="arial" font style="color: #FFFFFF">Photos</font>]
+*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Instructors_Feedbacks <font face="arial" font style="color: #FFFFFF">Feedbacks on our experience</font>]
+|}
+==The Team and Acknowledgements==
+{| class="wikitable" border="0" width="100%" cellpadding="10" cellspacing="1" style="padding: 5px; background-color: #ffffff; border: 2px solid #2171B8;text-align:center"
+!width="20%" align="left" valign="top" style="background:#2171B8; color:black"|
+<center>
+==<font face="arial" font style="color: #FFFFFF">Undergrads</font>==
+</center>
+*[http://openwetware.org/wiki/User:Cys <font face="arial" font style="color: #FFFFFF">Christin Sander</font>]
+*[http://openwetware.org/wiki/User:da104 <font face="arial" font style="color: #FFFFFF">Deepti Aswani</font>]
+*[http://openwetware.org/wiki/User:Farah <font face="arial" font style="color: #FFFFFF">Farah Vohra</font>]
+*[http://openwetware.org/wiki/User:Baijiongjun<font face="arial" font style="color: #FFFFFF"> Jiongjun Bai</font>]
+*[http://openwetware.org/wiki/User:Johnsy <font face="arial" font style="color: #FFFFFF">John Sy</font>]
+*[http://openwetware.org/wiki/User:JohnChattaway <font face="arial" font style="color: #FFFFFF">John Chattaway</font>]
+*[http://openwetware.org/wiki/User:Jonny<font face="arial" font style="color: #FFFFFF"> Jonathan Wells</font>]
+*[http://openwetware.org/wiki/User:Tom <font face="arial" font style="color: #FFFFFF">Tom Hinson</font>]
+!width="25%" align="left" valign="top"  color:black"|
+[[Image:IGEM GroupPhotos0015.JPG|thumb|center|200px|The Imperial College iGEM team and advisors on the terrace of the Biochemistry building]]
+!width="20%" align="left" valign="top" style="background:#2171B8; color:black"|
+<center>
+==<font face="arial" font style="color: #FFFFFF">Advisors</font>==
+</center>
+*[http://www3.imperial.ac.uk/people/r.kitney <font face="arial" font style="color: #FFFFFF">Prof. Richard Kitney</font>]
+*[http://www3.imperial.ac.uk/people/p.freemont <font face="arial" font style="color: #FFFFFF">Prof. Paul Freemont</font>]
+*[http://www3.imperial.ac.uk/people/d.mann <font face="arial" font style="color: #FFFFFF">Dr. David Mann</font>]
+*<font face="arial" font style="color: #FFFFFF">Kirsten Jensen</font>
+*[http://openwetware.org/wiki/User:Vincent <font face="arial" font style="color: #FFFFFF">Vincent Rouilly</font>]
+*[http://openwetware.org/wiki/User:chuehloo <font face="arial" font style="color: #FFFFFF">Chueh-Loo Poh</font>]
+*[http://openwetware.org/wiki/User:SxE00 <font face="arial" font style="color: #FFFFFF">Matthieu Bultelle</font>]
+!width="20%" align="left" valign="top" style="background:#2171B8; color:white"|
+<center>
+==<font face="arial" font style="color: #FFFFFF">Funding</font>==
+</center>
+*European Commission
+*Imperial College Deputy Rector's Fund
+*Imperial College Faculty of Engineering
+*Imperial College Faculty of Natural Sciences
+|}
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