Berkeley Work Schedule and Progress
From 2006.igem.org
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== Current Work: == | == Current Work: == | ||
*''Conjugation testing:''<br> | *''Conjugation testing:''<br> | ||
| - | ** | + | **oriT - Test plasmid mobility<br> |
| - | ** | + | **TraJf, TraJr - does promoter induction cause conjugation to initiate?<br> |
| - | ** | + | **Test mobilization construct<br> |
*''Construction:''<br> | *''Construction:''<br> | ||
| - | ** | + | **Spo0A - QuikChange (Stratagene kit site directed mutagenesis) out the Eco site and finish mobilizable construct for OriTf |
| - | *Other | + | *Lock and Key Strength |
| - | **- Find out cause for onRFP sporadic behaviour during conjugation (tetR presence?) | + | **Flourescence assays |
| + | *''Other'' | ||
| + | **onRFP - Find out cause for onRFP sporadic behaviour during conjugation (tetR presence?) | ||
== Milestones: == | == Milestones: == | ||
| - | + | ||
<i><u>November:</u></i><br> | <i><u>November:</u></i><br> | ||
Measurement data for constructs, construction continues | Measurement data for constructs, construction continues | ||
Latest revision as of 09:03, 14 November 2005
Current Work:
- Conjugation testing:
- oriT - Test plasmid mobility
- TraJf, TraJr - does promoter induction cause conjugation to initiate?
- Test mobilization construct
- oriT - Test plasmid mobility
- Construction:
- Spo0A - QuikChange (Stratagene kit site directed mutagenesis) out the Eco site and finish mobilizable construct for OriTf
- Lock and Key Strength
- Flourescence assays
- Other
- onRFP - Find out cause for onRFP sporadic behaviour during conjugation (tetR presence?)
Milestones:
November:
Measurement data for constructs, construction continues
September:
Project training continues, implementation begins
July-August:
Theoretical project design and background research on bacterial conjugation and technique training
May-June:
Synthetic Biology primer and project brainstorming
