Berkeley Work Schedule and Progress
From 2006.igem.org
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== Milestones: == | == Milestones: == | ||
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<i><u>November:</u></i><br> | <i><u>November:</u></i><br> | ||
Measurement data for constructs, construction continues | Measurement data for constructs, construction continues | ||
Latest revision as of 09:03, 14 November 2005
Current Work:
- Conjugation testing:
- oriT - Test plasmid mobility
- TraJf, TraJr - does promoter induction cause conjugation to initiate?
- Test mobilization construct
- oriT - Test plasmid mobility
- Construction:
- Spo0A - QuikChange (Stratagene kit site directed mutagenesis) out the Eco site and finish mobilizable construct for OriTf
- Lock and Key Strength
- Flourescence assays
- Other
- onRFP - Find out cause for onRFP sporadic behaviour during conjugation (tetR presence?)
Milestones:
November:
Measurement data for constructs, construction continues
September:
Project training continues, implementation begins
July-August:
Theoretical project design and background research on bacterial conjugation and technique training
May-June:
Synthetic Biology primer and project brainstorming
