Brown:Equipment needed

From 2006.igem.org

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Line 4: Line 4:
| type/size
| type/size
| quantity
| quantity
-
| Status
+
| Have(x)
 +
| Location
 +
| Comments
|-
|-
| '''Equipment'''
| '''Equipment'''
Line 17: Line 19:
| -80°C
| -80°C
| 1
| 1
 +
| we have space in a -80
 +
| JWW basement
|-  
|-  
|  
|  
| -20°C
| -20°C
| 1
| 1
 +
| yes
 +
| JWW228
|-  
|-  
|  
|  
| 4°C
| 4°C
| 1
| 1
 +
| yes
 +
| JWW228
|-  
|-  
-
| incubator  
+
| incubator (what kind?)
|  
|  
| 1
| 1
 +
| might work (heh)
 +
| JWW228
|-  
|-  
| pH meter
| pH meter
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|  
|  
| 1
| 1
 +
| we have access
 +
| JWW218
|-  
|-  
| cold bath
| cold bath
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|-  
|-  
| autoclave
| autoclave
-
|  
+
|
| 1
| 1
 +
| we have access
 +
|JWW 4th Floor
 +
|
 +
|-  
|-  
-
| cell shaker/incubator
+
| cell shaker inside 37 degree incubator
|  
|  
| 1
| 1
 +
| we have acess
 +
| JWW 3rd floor
|-  
|-  
| source of ddH2O
| source of ddH2O
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|-  
|-  
| microcentrifuge (w/temp cntrl.)
| microcentrifuge (w/temp cntrl.)
-
|  
+
| temp. cntrl not essential
| 1
| 1
|-  
|-  
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| 50mL and 15mL
| 50mL and 15mL
| 1
| 1
 +
| yes - 15 mL
 +
| very old, JWW228
|-  
|-  
| Electrophoresis gel set
| Electrophoresis gel set
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| 1
| 1
|-  
|-  
 +
| Manual pipettors / bulbs
 +
|
 +
| 2
 +
|-
| bunsen burner
| bunsen burner
|  
|  
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| 2
| 2
|-  
|-  
 +
| pasteur pipettes
 +
|
 +
| 1 box
 +
|-
| '''Gloves (boxes)'''
| '''Gloves (boxes)'''
|
|
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|
|
|-  
|-  
-
| centrifuge tubes
+
| eppendorf tubes
|  
|  
|  
|  
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|-  
|-  
|  
|  
-
| 200μL (PCR)
+
| 200μL (thin wall for PCR)
| 1000
| 1000
|-  
|-  
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|-  
|-  
| '''Reagents'''
| '''Reagents'''
 +
|
 +
|
|-  
|-  
| pH standard buffers for meter  
| pH standard buffers for meter  
|  
|  
| 1
| 1
 +
|-
 +
| 70% ethanol
 +
|
 +
|
|-  
|-  
-
|
+
| anitibiotics
 +
|
 +
|
 +
|-
 +
|
 +
| ampicillin
 +
|
 +
|-
 +
|
 +
| kanamycin
 +
|
 +
|-
 +
|
 +
| puromycin
 +
|
 +
|-
 +
| Agarose
 +
|
 +
|
 +
|-
 +
| Ethidium Bromide
 +
|
 +
|
 +
|-
 +
| tris[hydroxymethyl]aminomethane
 +
|
 +
|
 +
|-
 +
| Acetate
 +
|
 +
|
 +
|-
 +
| EDTA
 +
|
 +
|
 +
|-
 +
| Gel Loading Dye
 +
|
 +
|
 +
|-
 +
| DNA ladder
 +
|
 +
|
 +
|-
 +
| Restriction enzymes and Buffers
 +
|
 +
|
 +
|-
 +
|
 +
| EcoRI
 +
|
 +
|-
 +
|
 +
| SpeI
 +
|
 +
|-
 +
|
 +
| XbaI
 +
|
 +
|-
 +
|
 +
| PstI
 +
|
 +
|-
 +
| Taq Polymerase (Master Mix)
 +
|
 +
|
 +
|-
 +
| 100% ethanol
 +
|
 +
|
 +
|-
 +
| Socium Acetate
 +
|
 +
|
 +
|-
 +
| NaCl
 +
|
 +
|
 +
|-
 +
| T4 DNA ligase
 +
|
 +
|
 +
|-
 +
| T4 DNA ligase Buffer
 +
|
 +
|
 +
|-
 +
| liquid LB Broth
 +
|
 +
|
 +
|-
 +
| LB with agar
 +
|
 +
|
 +
|-
 +
| TSS Buffer
 +
|
 +
|
 +
|-
 +
| Mini-Prep Kit
 +
|
 +
|
 +
|-
 +
| Maxi-Prep Kit
 +
|
 +
|  
|-  
|-  
|}
|}

Latest revision as of 16:50, 19 June 2006

type/size quantity Have(x) Location Comments
Equipment
freezers/fridges
-80°C 1 we have space in a -80 JWW basement
-20°C 1 yes JWW228
4°C 1 yes JWW228
incubator (what kind?) 1 might work (heh) JWW228
pH meter 1
hot bath 1 we have access JWW218
cold bath 1
autoclave 1 we have access JWW 4th Floor
cell shaker inside 37 degree incubator 1 we have acess JWW 3rd floor
source of ddH2O 1
hot plates w/ stirring 2
stir bars (set) 1
PCR thermocycler 1
microcentrifuge (w/temp cntrl.) temp. cntrl not essential 1
centrifuge 50mL and 15mL 1 yes - 15 mL very old, JWW228
Electrophoresis gel set 4
Gel Power source 1
UV Gel viewer 1
UV Gel camera 1
Pipettemen
20μL 4
200μL 4
1000μL 4
Electronic pipettor can use 1-25mL pipettes 1
Manual pipettors / bulbs 2
bunsen burner 2
sparkers 2
wire loop to pick a bac clone 3
metal loop to spread bacteria 3
Glassware
Bottles
1L 4
500mL 8
100mL 8
Beakers
1L 2
250mL 8
Centrifuge tubes
50mL 100
15mL 100
Graduated cylinders

500mL

2
100mL 2
Electronic pipette tips (packages)
1mL 2
5mL 2
10mL 2
25mL 2
pasteur pipettes 1 box
Gloves (boxes)
small 1
medium 4
large 4
Plasticware
eppendorf tubes
1.5mL 2000
200μL (thin wall for PCR) 1000
Pipette tips
20μL a lot
200μL a lot
1000μL a lot
Bacterial cell culture plates 200
bacterial cell culture tubes (polypropylene) 100
tube racks/holders
for 1.5mL tubes 4
for 200μL tubes 2
Reagents
pH standard buffers for meter 1
70% ethanol
anitibiotics
ampicillin
kanamycin
puromycin
Agarose
Ethidium Bromide
tris[hydroxymethyl]aminomethane
Acetate
EDTA
Gel Loading Dye
DNA ladder
Restriction enzymes and Buffers
EcoRI
SpeI
XbaI
PstI
Taq Polymerase (Master Mix)
100% ethanol
Socium Acetate
NaCl
T4 DNA ligase
T4 DNA ligase Buffer
liquid LB Broth
LB with agar
TSS Buffer
Mini-Prep Kit
Maxi-Prep Kit
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