Recombinational Enahancer: Part BBa J3101
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These are the oligos that we ordered to make the recombinational enhancer. | These are the oligos that we ordered to make the recombinational enhancer. | ||
| + | *The underlined portion of T1 is an EcoRI sticky end | ||
| + | *The underlined portion of T3 is a PstI sticky end site | ||
| + | * The <font color=blue>dark blue</font color> portions of the top strand oligoes are the bio brick ends. | ||
| - | ' | + | 5'-->3' |
| - | ''''' | + | '''<font face="courier new"><font color=red>''T1:'' </font color> <font color=blue><u>AATTC</u>GCGGCCGCTTCTAGA</font color><font color=red>TTCGGGTGTCAACAATTGACCAAAATAT</font color> |
| - | ''''' | + | <font color=orange>'''''T2:''''' '''CGATTTACAGCGTAATGCGCTTTCTAGTGCAAATTGTGACCGC'''</font color> |
| - | ''''' | + | <font color=cyan>'''''T3:''''' '''ATTTT<font color=blue>ACTAGTTGCGGCCGCC<u>TGCA</u>'''''</font color> |
| - | '''''B2:''''' '''AGCGCATTACGCTGTAAATCGATATTTTGGTCAATTGTTGACACCCGAATCTAGAAGCGGCCGCG</font face>''' | + | </font color><font color=brown> '''''B1:''''' '''GGCGGCCGCAACTAGTAAAATGCGGTCACAATTTGCACTAGAA'''</font color> |
| + | |||
| + | <font color= purple>'''''B2:''''' '''AGCGCATTACGCTGTAAATCGATATTTTGGTCAATTGTTGACACCCGAATCTAGAAGCGGCCGCG</font color></font face>''' | ||
| + | |||
| + | |||
| + | <font face="courier new"><font size =2><font color=red>AATTCGCGGCCGCTTCTAGATTCGGGTGTCAACAATTGACCAAAATAT</font color><font color=orange>CGATTTACAGCGTAATGCGCTTTCTAGTGCAAATTGTGACCGC</font color><font color=cyan>ATTTTACTAGTTGCGGCCGCCTGCA</font color> | ||
| + | |||
| + | <font color=purple> GCGCCGGCGAAGATCTAAGCCCACAGTTGTTAACTGGTTTTATAGCTAAATGTCGCATTACGCGA</font color><font color=brown>AAGATCACGTTTAACACTGGCGTAAAATGATCAACGCCGGCGG</font color></font size></font face> | ||
Latest revision as of 14:49, 6 July 2006
Recombinational Enhancer: Part BBa_J3101[http://partsregistry.org/Part:BBa_J3101]
These are the oligos that we ordered to make the recombinational enhancer.
- The underlined portion of T1 is an EcoRI sticky end
- The underlined portion of T3 is a PstI sticky end site
- The dark blue portions of the top strand oligoes are the bio brick ends.
5'-->3'
T1: AATTCGCGGCCGCTTCTAGATTCGGGTGTCAACAATTGACCAAAATAT
T2: CGATTTACAGCGTAATGCGCTTTCTAGTGCAAATTGTGACCGC
T3: ATTTTACTAGTTGCGGCCGCCTGCA
B1: GGCGGCCGCAACTAGTAAAATGCGGTCACAATTTGCACTAGAA
B2: AGCGCATTACGCTGTAAATCGATATTTTGGTCAATTGTTGACACCCGAATCTAGAAGCGGCCGCG
AATTCGCGGCCGCTTCTAGATTCGGGTGTCAACAATTGACCAAAATATCGATTTACAGCGTAATGCGCTTTCTAGTGCAAATTGTGACCGCATTTTACTAGTTGCGGCCGCCTGCA
GCGCCGGCGAAGATCTAAGCCCACAGTTGTTAACTGGTTTTATAGCTAAATGTCGCATTACGCGAAAGATCACGTTTAACACTGGCGTAAAATGATCAACGCCGGCGG
