Recombinational Enahancer: Part BBa J3101
From 2006.igem.org
(Difference between revisions)
Fizzle6821 (Talk | contribs) |
Fizzle6821 (Talk | contribs) |
| (One intermediate revision not shown) | |
Latest revision as of 14:49, 6 July 2006
Recombinational Enhancer: Part BBa_J3101[http://partsregistry.org/Part:BBa_J3101]
These are the oligos that we ordered to make the recombinational enhancer.
- The underlined portion of T1 is an EcoRI sticky end
- The underlined portion of T3 is a PstI sticky end site
- The dark blue portions of the top strand oligoes are the bio brick ends.
5'-->3'
T1: AATTCGCGGCCGCTTCTAGATTCGGGTGTCAACAATTGACCAAAATAT
T2: CGATTTACAGCGTAATGCGCTTTCTAGTGCAAATTGTGACCGC
T3: ATTTTACTAGTTGCGGCCGCCTGCA
B1: GGCGGCCGCAACTAGTAAAATGCGGTCACAATTTGCACTAGAA
B2: AGCGCATTACGCTGTAAATCGATATTTTGGTCAATTGTTGACACCCGAATCTAGAAGCGGCCGCG
AATTCGCGGCCGCTTCTAGATTCGGGTGTCAACAATTGACCAAAATATCGATTTACAGCGTAATGCGCTTTCTAGTGCAAATTGTGACCGCATTTTACTAGTTGCGGCCGCCTGCA
GCGCCGGCGAAGATCTAAGCCCACAGTTGTTAACTGGTTTTATAGCTAAATGTCGCATTACGCGAAAGATCACGTTTAACACTGGCGTAAAATGATCAACGCCGGCGG
