Berkeley Work Schedule and Progress
From 2006.igem.org
(Difference between revisions)
| Line 6: | Line 6: | ||
*''Construction:''<br> | *''Construction:''<br> | ||
**Spo0A - QuikChange (Stratagene kit site directed mutagenesis) out the Eco site and finish mobilizable construct for OriTf | **Spo0A - QuikChange (Stratagene kit site directed mutagenesis) out the Eco site and finish mobilizable construct for OriTf | ||
| + | *Lock and Key Strength | ||
| + | **Flourescence assays | ||
*''Other'' | *''Other'' | ||
**onRFP - Find out cause for onRFP sporadic behaviour during conjugation (tetR presence?) | **onRFP - Find out cause for onRFP sporadic behaviour during conjugation (tetR presence?) | ||
Revision as of 08:56, 14 November 2005
Current Work:
- Conjugation testing:
- oriT - Test plasmid mobility
- TraJf, TraJr - does promoter induction cause conjugation to initiate?
- Test mobilization construct
- oriT - Test plasmid mobility
- Construction:
- Spo0A - QuikChange (Stratagene kit site directed mutagenesis) out the Eco site and finish mobilizable construct for OriTf
- Lock and Key Strength
- Flourescence assays
- Other
- onRFP - Find out cause for onRFP sporadic behaviour during conjugation (tetR presence?)
Milestones:
November:
Measurement data for constructs, construction continues
September:
Project training continues, implementation begins
July-August:
Theoretical project design and background research on bacterial conjugation and technique training
May-June:
Synthetic Biology primer and project brainstorming
