Recombinational Enahancer: Part BBa J3101

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<font face="courier new"><font size =2><font color=red>AATTCGCGGCCGCTTCTAGATTCGGGTGTCAACAATTGACCAAAATAT</font color><font color=orange>CGATTTACAGCGTAATGCGCTTTCTAGTGCAAATTGTGACCGC</font color><font color=cyan>ATTTTACTAGTTGCGGCCGCCTGCA</font color>
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<font face="courier new"><font size =1><font color=red>AATTCGCGGCCGCTTCTAGATTCGGGTGTCAACAATTGACCAAAATAT</font color><font color=orange>CGATTTACAGCGTAATGCGCTTTCTAGTGCAAATTGTGACCGC</font color><font color=cyan>ATTTTACTAGTTGCGGCCGCCTGCA</font color>
&nbsp;&nbsp;&nbsp;<font color=purple> GCGCCGGCGAAGATCTAAGCCCACAGTTGTTAACTGGTTTTATAGCTAAATGTCGCATTACGCGA</font color><font color=brown>AAGATCACGTTTAACACTGGCGTAAAATGATCAACGCCGGCGG</font color></font size></font face>
&nbsp;&nbsp;&nbsp;<font color=purple> GCGCCGGCGAAGATCTAAGCCCACAGTTGTTAACTGGTTTTATAGCTAAATGTCGCATTACGCGA</font color><font color=brown>AAGATCACGTTTAACACTGGCGTAAAATGATCAACGCCGGCGG</font color></font size></font face>

Revision as of 14:49, 6 July 2006

Recombinational Enhancer: Part BBa_J3101[http://partsregistry.org/Part:BBa_J3101]

These are the oligos that we ordered to make the recombinational enhancer.

  • The underlined portion of T1 is an EcoRI sticky end
  • The underlined portion of T3 is a PstI sticky end site
  • The dark blue portions of the top strand oligoes are the bio brick ends.

5'-->3'

T1: AATTCGCGGCCGCTTCTAGATTCGGGTGTCAACAATTGACCAAAATAT

T2: CGATTTACAGCGTAATGCGCTTTCTAGTGCAAATTGTGACCGC

T3: ATTTTACTAGTTGCGGCCGCCTGCA

B1: GGCGGCCGCAACTAGTAAAATGCGGTCACAATTTGCACTAGAA

B2: AGCGCATTACGCTGTAAATCGATATTTTGGTCAATTGTTGACACCCGAATCTAGAAGCGGCCGCG


AATTCGCGGCCGCTTCTAGATTCGGGTGTCAACAATTGACCAAAATATCGATTTACAGCGTAATGCGCTTTCTAGTGCAAATTGTGACCGCATTTTACTAGTTGCGGCCGCCTGCA

    GCGCCGGCGAAGATCTAAGCCCACAGTTGTTAACTGGTTTTATAGCTAAATGTCGCATTACGCGAAAGATCACGTTTAACACTGGCGTAAAATGATCAACGCCGGCGG

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