Recombinational Enahancer: Part BBa J3101
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| - | <font face="courier new"><font size = | + | <font face="courier new"><font size =1><font color=red>AATTCGCGGCCGCTTCTAGATTCGGGTGTCAACAATTGACCAAAATAT</font color><font color=orange>CGATTTACAGCGTAATGCGCTTTCTAGTGCAAATTGTGACCGC</font color><font color=cyan>ATTTTACTAGTTGCGGCCGCCTGCA</font color> |
<font color=purple> GCGCCGGCGAAGATCTAAGCCCACAGTTGTTAACTGGTTTTATAGCTAAATGTCGCATTACGCGA</font color><font color=brown>AAGATCACGTTTAACACTGGCGTAAAATGATCAACGCCGGCGG</font color></font size></font face> | <font color=purple> GCGCCGGCGAAGATCTAAGCCCACAGTTGTTAACTGGTTTTATAGCTAAATGTCGCATTACGCGA</font color><font color=brown>AAGATCACGTTTAACACTGGCGTAAAATGATCAACGCCGGCGG</font color></font size></font face> | ||
Revision as of 14:49, 6 July 2006
Recombinational Enhancer: Part BBa_J3101[http://partsregistry.org/Part:BBa_J3101]
These are the oligos that we ordered to make the recombinational enhancer.
- The underlined portion of T1 is an EcoRI sticky end
- The underlined portion of T3 is a PstI sticky end site
- The dark blue portions of the top strand oligoes are the bio brick ends.
5'-->3'
T1: AATTCGCGGCCGCTTCTAGATTCGGGTGTCAACAATTGACCAAAATAT
T2: CGATTTACAGCGTAATGCGCTTTCTAGTGCAAATTGTGACCGC
T3: ATTTTACTAGTTGCGGCCGCCTGCA
B1: GGCGGCCGCAACTAGTAAAATGCGGTCACAATTTGCACTAGAA
B2: AGCGCATTACGCTGTAAATCGATATTTTGGTCAATTGTTGACACCCGAATCTAGAAGCGGCCGCG
AATTCGCGGCCGCTTCTAGATTCGGGTGTCAACAATTGACCAAAATATCGATTTACAGCGTAATGCGCTTTCTAGTGCAAATTGTGACCGCATTTTACTAGTTGCGGCCGCCTGCA
GCGCCGGCGAAGATCTAAGCCCACAGTTGTTAACTGGTTTTATAGCTAAATGTCGCATTACGCGAAAGATCACGTTTAACACTGGCGTAAAATGATCAACGCCGGCGG
