Brown:Projects
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[[Brown:iGEM portal 2006 |Home]] | [[Brown:iGEM portal 2006 |Home]] | ||
| + | == Bacterial Freeze Tag == | ||
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== Magnetotactic Bacteria == | == Magnetotactic Bacteria == | ||
| - | + | '''Avadin-Biotin Complex''' | |
To create magnetosomes covered in Avadin. The basic idea is to create a fusion protein from MagA and Avadin, then isolate the magnetic particles, which will now be coated in avadin. Avadin forms one of the strongest bindings known with the molecule biotin. | To create magnetosomes covered in Avadin. The basic idea is to create a fusion protein from MagA and Avadin, then isolate the magnetic particles, which will now be coated in avadin. Avadin forms one of the strongest bindings known with the molecule biotin. | ||
Revision as of 18:37, 6 July 2006
Bacterial Freeze Tag
Magnetotactic Bacteria
Avadin-Biotin Complex
To create magnetosomes covered in Avadin. The basic idea is to create a fusion protein from MagA and Avadin, then isolate the magnetic particles, which will now be coated in avadin. Avadin forms one of the strongest bindings known with the molecule biotin.
Bayer, E. A. and Wilchek, M. (1978). The avidin-biotin complex as a tool in molecular biology. Trends Biochem. Sci. 3, N237-N239.
http://www.weizmann.ac.il/Biological_Chemistry/scientist/Bayer/BayerAvidin.html great review
http://www.avidity.com/apps.html current applications of tagging biotin
http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=92791044 Artifical synthesized Gene coding for avidin
