Timeline
From 2006.igem.org
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==<font color='red'>Week 4: 10<sup>th</sup> - 14<sup>th</sup> July</font>== | ==<font color='red'>Week 4: 10<sup>th</sup> - 14<sup>th</sup> July</font>== | ||
| - | #Locate parts in the registry for the modelling of the arsenic biosensor and the 3D structure builder | + | #Locate parts in the registry for the modelling of the arsenic biosensor and the 3D structure builder |
#Continuing in the lab with putting the arsR and new lacZ genes into biobrick constructs and trying to solve issues with isolating DNA fragments from gels | #Continuing in the lab with putting the arsR and new lacZ genes into biobrick constructs and trying to solve issues with isolating DNA fragments from gels | ||
#Devising new pH experiments to calibrate the sensor | #Devising new pH experiments to calibrate the sensor | ||
Revision as of 14:14, 11 July 2006
Contents |
Week 4: 10th - 14th July
- Locate parts in the registry for the modelling of the arsenic biosensor and the 3D structure builder
- Continuing in the lab with putting the arsR and new lacZ genes into biobrick constructs and trying to solve issues with isolating DNA fragments from gels
- Devising new pH experiments to calibrate the sensor
- Badge making, postcard making, locating possible people to send them to
- Organising Cambridge trip
- Initial modelling of the biosensor, finding out all the reactions involved and turning them into equations
Week 3: 3rd - 7th July
Objectives for the arsenic sensor:
Assess the effects of the:
- 1) bacterial population density on pH
- 2) growth phase on pH change
- 3) concentration of lactose on pH
- 4) time on pH
All effects are to be measured on the pH of the liquid cultures with the Lacz deficient strain of E-coli.
Due to problems in the lab, the above objectives are revised to next week. These problems are illustrated in the [http://2006.igem.org/Lab_Work Lab work section]. Objectives for this week are now to get the first PCR primers made to assemble our first biobricks. Tests for the production of lactic acid on L-agar plates using the LacZ plasmids should be started tomorrow afternoon after the lecture from Dr Elfick.
Objectives for the 3d structure builder:
1) Determine whether to use the Urease or Phosphatase enzyme to precipitate a solid substrate
Week 2: 26th - 30th June
More Brainstorming
Week 1: 19th - 23rd June
Brainstorming
[http://2006.igem.org/University_of_Edinburgh_2006 Main page]
