Aromatic Bacteria : Chiba 2006

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Line 41: Line 41:
[http://en.wikipedia.org/wiki/Limonene Limonene]<br>
[http://en.wikipedia.org/wiki/Limonene Limonene]<br>
[http://en.wikipedia.org/wiki/Pinene Pinene]<br>
[http://en.wikipedia.org/wiki/Pinene Pinene]<br>
-
[http://en.wikipedia.org/wiki/Linarool Linarool]<br>
+
[http://en.wikipedia.org/wiki/Linalool Linalool]<br>
[http://en.wikipedia.org/wiki/Geraniol Geraniol]<br>
[http://en.wikipedia.org/wiki/Geraniol Geraniol]<br>
[http://en.wikipedia.org/wiki/Farnesene Farnesene]<br>
[http://en.wikipedia.org/wiki/Farnesene Farnesene]<br>

Revision as of 14:29, 6 September 2006

Contents

concept

Aromaken4.jpg

















Wow!! By lighting bacteria, a refreshing scent stars filling the room. In the dark, the light sensing E.coli will turn orenge. With the red light on, the color of E.coli fades out, and aroma is produced instead.

members

schedule

            9/7   8   9   10  11  12  13  14  15  16   17  18
Daisuke                
Kensuke                                         
Mayu
Shizuka
Keiichi        


How it works

How it works.jpg


aroma

Firstly,we are going to make E.coli that smell good!

We are going to get DNA that codes these products synthases.
[http://en.wikipedia.org/wiki/Carene Carene]
[http://en.wikipedia.org/wiki/Limonene Limonene]
[http://en.wikipedia.org/wiki/Pinene Pinene]
[http://en.wikipedia.org/wiki/Linalool Linalool]
[http://en.wikipedia.org/wiki/Geraniol Geraniol]
[http://en.wikipedia.org/wiki/Farnesene Farnesene]




light receptor

Secondary,We are going to make a light receptor that react different wavelength.

PCB reacts redlight (that is about 660nm). If we change PCB to PΦB and PEB , the absorption wavelength shifts 15nm respectively.
Light receptor.jpg


carotenoid

agenda

  • 9/5--We solved a problem yesterday. PUC-EBI was very dirty so it caused a lot of problem. After cleaning it, We got a clean band of PUC-EBI(linear).
  • 9/4--We want to make PUC-EBIY. We got EBIY (it's orange color),but couldn't get PUC(vector).A band of PUC-EBI(linear)was very weak ,and we could see several bands. So we discussed what caused this problem.
  • 8/29--We discussed about the light sensor bacteria.
  • 8/12--Our first experiment using an aroma oil(Sweet Flavor-smells like orange). 10μl of oil was necessary for LB medium(solid and liquid) to smell good, or at least, better.
  • 8/4 -- We created parts J29021(generator-3 enzymes that is used to produce a lycopene) and J29022(device-light receptor and LacI).
  • 8/4 -- We added EuY(J29030) and carene cyclase(J29036) to the Registry. 4 changes were made to carene cyclase to remove restriction sites of ecoR1 and pst1.
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