Berkeley Work Schedule and Progress
From 2006.igem.org
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== Current Work: == | == Current Work: == | ||
| - | ''Conjugation testing:''<br> | + | #''Conjugation testing:''<br> |
| - | - oriT - Test plasmid mobility<br> | + | ##- oriT - Test plasmid mobility<br> |
- TraJf, TraJr - does promoter induction cause conjugation to initiate?<br> | - TraJf, TraJr - does promoter induction cause conjugation to initiate?<br> | ||
- Test mobilization construct<br> | - Test mobilization construct<br> | ||
| - | ''Construction:''<br> | + | #''Construction:''<br> |
| - | - Spo0A - QuikChange (Stratagene kit site directed mutagenesis) out the Eco site and finish mobilizable construct for OriTf | + | ##- Spo0A - QuikChange (Stratagene kit site directed mutagenesis) out the Eco site and finish mobilizable construct for OriTf |
| - | Other | + | #Other |
- Find out cause for onRFP sporadic behaviour during conjugation (tetR presence?) | - Find out cause for onRFP sporadic behaviour during conjugation (tetR presence?) | ||
Revision as of 08:52, 14 November 2005
Current Work:
- Conjugation testing:
- - oriT - Test plasmid mobility
- - oriT - Test plasmid mobility
- TraJf, TraJr - does promoter induction cause conjugation to initiate?
- Test mobilization construct
- Construction:
- - Spo0A - QuikChange (Stratagene kit site directed mutagenesis) out the Eco site and finish mobilizable construct for OriTf
- Other
- Find out cause for onRFP sporadic behaviour during conjugation (tetR presence?)
Milestones:
November:
Measurement data for constructs, construction continues
September:
Project training continues, implementation begins
July-August:
Theoretical project design and background research on bacterial conjugation and technique training
May-June:
Synthetic Biology primer and project brainstorming
