Berkeley Work Schedule and Progress
From 2006.igem.org
(Difference between revisions)
| Line 2: | Line 2: | ||
#''Conjugation testing:''<br> | #''Conjugation testing:''<br> | ||
##- oriT - Test plasmid mobility<br> | ##- oriT - Test plasmid mobility<br> | ||
| - | - TraJf, TraJr - does promoter induction cause conjugation to initiate?<br> | + | ##- TraJf, TraJr - does promoter induction cause conjugation to initiate?<br> |
| - | - Test mobilization construct<br> | + | ##- Test mobilization construct<br> |
#''Construction:''<br> | #''Construction:''<br> | ||
##- Spo0A - QuikChange (Stratagene kit site directed mutagenesis) out the Eco site and finish mobilizable construct for OriTf | ##- Spo0A - QuikChange (Stratagene kit site directed mutagenesis) out the Eco site and finish mobilizable construct for OriTf | ||
#Other | #Other | ||
| - | - Find out cause for onRFP sporadic behaviour during conjugation (tetR presence?) | + | ##- Find out cause for onRFP sporadic behaviour during conjugation (tetR presence?) |
Revision as of 08:53, 14 November 2005
Current Work:
- Conjugation testing:
- - oriT - Test plasmid mobility
- - TraJf, TraJr - does promoter induction cause conjugation to initiate?
- - Test mobilization construct
- - oriT - Test plasmid mobility
- Construction:
- - Spo0A - QuikChange (Stratagene kit site directed mutagenesis) out the Eco site and finish mobilizable construct for OriTf
- Other
- - Find out cause for onRFP sporadic behaviour during conjugation (tetR presence?)
Milestones:
November:
Measurement data for constructs, construction continues
September:
Project training continues, implementation begins
July-August:
Theoretical project design and background research on bacterial conjugation and technique training
May-June:
Synthetic Biology primer and project brainstorming
