Berkeley Work Schedule and Progress
From 2006.igem.org
(Difference between revisions)
| Line 6: | Line 6: | ||
*''Construction:''<br> | *''Construction:''<br> | ||
**- Spo0A - QuikChange (Stratagene kit site directed mutagenesis) out the Eco site and finish mobilizable construct for OriTf | **- Spo0A - QuikChange (Stratagene kit site directed mutagenesis) out the Eco site and finish mobilizable construct for OriTf | ||
| - | *Other | + | *''Other'' |
**- Find out cause for onRFP sporadic behaviour during conjugation (tetR presence?) | **- Find out cause for onRFP sporadic behaviour during conjugation (tetR presence?) | ||
Revision as of 08:54, 14 November 2005
Current Work:
- Conjugation testing:
- - oriT - Test plasmid mobility
- - TraJf, TraJr - does promoter induction cause conjugation to initiate?
- - Test mobilization construct
- - oriT - Test plasmid mobility
- Construction:
- - Spo0A - QuikChange (Stratagene kit site directed mutagenesis) out the Eco site and finish mobilizable construct for OriTf
- Other
- - Find out cause for onRFP sporadic behaviour during conjugation (tetR presence?)
Milestones:
November:
Measurement data for constructs, construction continues
September:
Project training continues, implementation begins
July-August:
Theoretical project design and background research on bacterial conjugation and technique training
May-June:
Synthetic Biology primer and project brainstorming
